Identification of human blood messenger ribonucleic acids through non-polymerase chain reaction based multiplexing.

Abstract

The goal of this research project was to develop a method for the identification of human blood that was simple and fast to use, yet sensitive and specific enough for forensic casework. Based on these criteria, the ideal assay would be based on messenger ribonucleic acid (mRNA) multiplexing specific for blood identification. According to the central dogma of molecular biology and gene expression, it can be theorized that the identity of human specific biological material can be obtained based only on the mRNA expressed by genes of a particular tissue. Once isolated, the tissue specific mRNA can be detected using the affinity of deoxyribonucleic acid (DNA) probes which contain the complementary sequence for annealing. The newly annealed double stranded nucleic acid will then serve as the target for detection via fluorescent molecular labeling, which will allow the human specific biological material targets to be detectable under ultraviolet light. Development of such a method would provide the field with a more rapid and accurate assay for analysis of forensic serology samples