This study investigates whether different masculine identities affect the relationship between masculinity threat and threat response in an organizational context. Specifically, the study seeks to extend the extant literature regarding the threat men often perceive regarding their own masculinity when subordinate to a female, as well as examine how masculinity contest cultures can exacerbate these perceptions while fatherhood primes may alleviate them. Fatherhood is an equality masculinity that promotes egalitarian gender relations and contrasts with the prevalent traditional masculinity which is a hegemonic masculinity is built on feminine subordination. Data trended in the hypothesized direction, which indicated that masculinity threat responses were lower for participants who received the fatherhood prime; due to sampling issues, however, statistical significance was not reached. The general direction of the data, albeit not statistically significant, was also congruent with past findings that female work superiors elicit greater threat responses from men than male superiors do. The general direction of the data was also indicative of a relationship between masculinity contest culture and masculinity threat responses even though the hypothesis was not statistically supported. Overall, the study results indicate replication with a larger sample obtained from an organizational context is warranted.