dc.contributor.author | Nambiar, Nayna | |
dc.contributor.author | Tyagi, Shrea | |
dc.contributor.author | Reddig, B. J. | |
dc.contributor.author | Litt, P. | |
dc.contributor.author | Jaroni, D. | |
dc.contributor.author | Blewett, E. L. | |
dc.date.accessioned | 2020-05-05T19:37:52Z | |
dc.date.available | 2020-05-05T19:37:52Z | |
dc.date.issued | 2020-02-28 | |
dc.identifier | ouhd_nambiar_cloningandsequencingofthe_2020 | |
dc.identifier.citation | Nambiar, N., Tyagi, S., Reddig, B. J., Litt, P., Jaroni, D., & Blewett, E. L. (2020, Feb. 28). Cloning and sequencing of the depolymerase-like gene from Bacteriophage J25. Poster presented at Research Day at Oklahoma State University Center for Health Sciences, Tulsa, OK. | |
dc.identifier.uri | https://hdl.handle.net/11244/324227 | |
dc.description.abstract | Bacteriophage are viruses that infect, replicate and kill bacteria. Salmonella and EHEC food poisoning are caused by Salmonella and E. coli bacteria. Bacteriophage can be used to prevent food poisoning by application to food products or processing machinery. Bacteriophage J25 specifically infects Salmonella and E. coli bacteria. We cloned fragments of the J25 genome, sequence the DNA and used bioinformatics to identify J25. We used genome data from similar bacteriophage in Genbank to design primers to amplify the depolymerase-like gene. We amplified and cloned this gene. When expressed, the gene product will be test with bacteriophage food treatment where it should augment bacteriophage killing. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | Oklahoma State University Center for Health Services | |
dc.rights | The author(s) retain the copyright of have the right to deposit the item giving the Oklahoma State University Library a limited, non-exclusive right to share this material in its institutional repository. Contact Digital Resources and Discovery Services at lib-dis@okstate.edu or 405-744-9161 for the permission policy on the use, reproduction or distribution of this material. | |
dc.title | Cloning and sequencing of the depolymerase-like gene from Bacteriophage J25 | |
osu.filename | ouhd_nambiar_cloningandsequencingofthe_2020.pdf | |
dc.type.genre | Presentation | |
dc.type.material | Text | |
dc.subject.keywords | bacteriophage | |
dc.subject.keywords | cloning | |
dc.subject.keywords | depolymerase | |
dc.subject.keywords | salmonella | |