dc.contributor.author | Iyer, Janaki K. | |
dc.contributor.author | Kalra, Mamta | |
dc.contributor.author | Kaul, Anil | |
dc.contributor.author | Payton, Mark E. | |
dc.contributor.author | Kaul, Rashmi | |
dc.date.accessioned | 2019-08-21T22:01:22Z | |
dc.date.available | 2019-08-21T22:01:22Z | |
dc.date.issued | 2017-10-07 | |
dc.identifier | oksd_iyer_estrogenrecepto_2017 | |
dc.identifier.citation | Iyer, J. K., Kalra, M., Kaul, A., Payton, M. E., & Kaul, R. (2017). Estrogen receptor expression in chronic hepatitis C and hepatocellular carcinoma pathogenesis. World Journal of Gastroenterology, 23(37), 6802-6816. https://doi.org/10.3748/wjg.v23.i37.6802 | |
dc.identifier.uri | https://hdl.handle.net/11244/321184 | |
dc.description.abstract | AIM: To investigate gender-specific liver estrogen receptor (ER) expression in normal subjects and patients with hepatitis C virus (HCV)-related cirrhosis and hepatocellular carcinoma (HCC). | |
dc.description.abstract | METHODS: Liver tissues from normal donors and patients diagnosed with HCV-related cirrhosis and HCV-related HCC were obtained from the NIH Liver Tissue and Cell Distribution System. The expression of ER subtypes, ERa and ERB, were evaluated by Western blotting and real-time RT-PCR. The subcellular distribution of ERa and ERB was further determined in nuclear and cytoplasmic tissue lysates along with the expression of inflammatory [activated NF-KB and IKB-kinase (IKK)] and oncogenic (cyclin D1) markers by Western blotting and immunohistochemistry. The expression of ERa and ERB was correlated with the expression of activated NF-KB, activated IKK and cyclin D1 by Spearman's correlation. | |
dc.description.abstract | RESULTS: Both ER subtypes were expressed in normal livers but male livers showed significantly higher expression of ERa than females (P < 0.05). We observed significantly higher mRNA expression of ERa in HCV-related HCC liver tissues as compared to normals (P < 0.05) and ERB in livers of HCV-related cirrhosis and HCV-related HCC subjects (P < 0.05). At the protein level, there was a significantly higher expression of nuclear ERa in livers of HCV-related HCC patients and nuclear ERB in HCV-related cirrhosis patients as compared to normals (P < 0.05). Furthermore, we observed a significantly higher expression of phosphorylated NF-KB and cyclin D1 in diseased livers (P < 0.05). There was a positive correlation between the expression of nuclear ER subtypes and nuclear cyclin D1 and a negative correlation between cytoplasmic ER subtypes and cytoplasmic phosphorylated IKK in HCV-related HCC livers. These findings suggest that dysregulated expression of ER subtypes following chronic HCV-infection may contribute to the progression of HCV-related cirrhosis to HCV-related HCC. | |
dc.description.abstract | CONCLUSION: Gender differences were observed in ERa expression in normal livers. Alterations in ER subtype expression observed in diseased livers may influence gender-related disparity in HCV-related pathogenesis. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | Baishideng Publishing Group | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Estrogen receptor expression in chronic hepatitis C and hepatocellular carcinoma pathogenesis | |
osu.filename | oksd_iyer_estrogenrecepto_2017.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.3748/wjg.v23.i37.6802 | |
dc.description.department | Biochemistry and Microbiology | |
dc.description.department | Health Care Administration | |
dc.description.department | Statistics | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | estrogen receptor alpha | |
dc.subject.keywords | estrogen receptor beta | |
dc.subject.keywords | hepatitis c virus-related cirrhosis | |
dc.subject.keywords | hepatitis c virus-related hepatocellular carcinoma | |
dc.subject.keywords | sex and gender | |
dc.subject.keywords | normal liver | |