Urine Drug Testing Via Volumetric Absorptive Microsampling Tips
MetadataShow full item record
Presently, laboratories typically perform urine drug testing with collected urine specimens using the dilute and shoot extraction technique. While the dilute and shoot technique is a functional means of evaluation for drugs, the development of Volumetric Absorptive Microsampling (VAMS) may improve the process of drug testing urine samples. This research compared urine analysis using dilute and shoot extraction to that of dried VAMS tips for drugs using liquid chromatography - tandem mass spectrometry (LC-MS/MS). Most drugs included in the panel were from the benzodiazepine and opioid class. The first element of evaluation was the ability to recover glucuronides. A hydrolysis method was devised that extracted the tips with methanol, followed by dry down and resuspension in enzyme and buffer, allowing for glucuronide conversion to parent drug and subsequent analysis. Following the development of the method, it was applied to anonymized specimens and the results were compared with a clinically validated dilute and shoot assay. It was determined that the techniques provided qualitatively similar results, suggesting that with appropriate validation, the VAMS methodology may be suitable for clinical analysis of drugs in urine specimens.
- OSU Theses