Show simple item record

Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris

dc.contributor.authorCanales, Mario
dc.contributor.authorPerez de la Lastra, Jose M.
dc.contributor.authorNaranjo, Victoria
dc.contributor.authorNijhof, Ard M.
dc.contributor.authorHope, Michelle
dc.contributor.authorJongejan, Frans
dc.contributor.authorde la Fuente, Jose
dc.date.accessioned2018-11-09T21:11:03Z
dc.date.available2018-11-09T21:11:03Z
dc.date.issued2008-02-14
dc.identifieroksd_canales_expressionofre_2008
dc.identifier.citationCanales, M., Perez de la Lastra, J. M., Naranjo, V., Nijhof, A. M., Hope, M., Jongejan, F., & de la Fuente, J. (2008). Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris. BMC Biotechnology, 8, Article 14. https://doi.org/10.1186/1472-6750-8-14
dc.identifier.urihttps://hdl.handle.net/11244/302087
dc.description.abstractBackground: Rhipicephalus (Boophilus) spp. ticks economically impact on cattle production in Africa and other tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The R. microplus Bm86 protective antigen has been produced by recombinant DNA technology and shown to protect cattle against tick infestations.
dc.description.abstractResults: In this study, the genes for Bm86 (R. microplus), Ba86 (R. annulatus) and Bd86 (R. decoloratus) were cloned and characterized from African or Asian tick strains and the recombinant proteins were secreted and purified from P. pastoris. The secretion of recombinant Bm86 ortholog proteins in P. pastoris allowed for a simple purification process rendering a final product with high recovery (35 - 42%) and purity (80 - 85%) and likely to result in a more reproducible conformation closely resembling the native protein. Rabbit immunization experiments with recombinant proteins showed immune cross-reactivity between Bm86 ortholog proteins.
dc.description.abstractConclusion: These experiments support the development and testing of vaccines containing recombinant Bm86, Ba86 and Bd86 secreted in P. pastoris for the control of tick infestations in Africa.
dc.formatapplication/pdf
dc.languageen_US
dc.publisherBioMed Central
dc.rightsThis material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information.
dc.titleExpression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris
osu.filenameoksd_canales_expressionofre_2008.pdf
dc.description.peerreviewPeer reviewed
dc.identifier.doi10.1186/1472-6750-8-14
dc.description.departmentVeterinary Pathobiology
dc.type.genreArticle
dc.type.materialText
dc.subject.keywordsrecombinant protein
dc.subject.keywordstick infestation
dc.subject.keywordsmethanol induction
dc.subject.keywordsGS115 strain
dc.subject.keywordspastoris strain GS115


Files in this item

Thumbnail
Name:
oksd_canales_expressionofre_20 ...
Size:
392.1Kb
Format:
PDF
View/Open

This item appears in the following Collection(s)

Show simple item record