dc.contributor.author | Zeng, Xiangming | |
dc.contributor.author | Huang, Chaoqun | |
dc.contributor.author | Senavirathna, Lakmini | |
dc.contributor.author | Wang, Pengcheng | |
dc.contributor.author | Liu, Lin | |
dc.date.accessioned | 2018-11-09T21:11:02Z | |
dc.date.available | 2018-11-09T21:11:02Z | |
dc.date.issued | 2017-01-17 | |
dc.identifier | oksd_zeng_mir-27binhibits_2017 | |
dc.identifier.citation | Zeng, X., Huang, C., Senavirathna, L., Wang, P., & Liu, L. (2017). miR-27b inhibits fibroblast activation via targeting TGFB signaling pathway. BMC Cell Biology, 18, Article 9. https://doi.org/10.1186/s12860-016-0123-7 | |
dc.identifier.uri | https://hdl.handle.net/11244/302085 | |
dc.description.abstract | Background: MicroRNAs are a group of small RNAs that regulate gene expression at the posttranscriptional level. They regulate almost every aspect of cellular processes. In this study, we investigated whether miR-27b regulates pulmonary fibroblast activation. | |
dc.description.abstract | Results: We found that miR-27b was down-regulated in fibrotic lungs and fibroblasts from an experimental mouse model of pulmonary fibrosis. The overexpression of miR-27b with a lentiviral vector inhibited TGFB1-stimulated mRNA expression of collagens (COL1A1, COL3A1, and COL4A1) and alpha-smooth muscle actin, and protein expression of Col3A1 and alpha-smooth muscle actin in LL29 human pulmonary fibroblasts. miR-27b also reduced contractile activity of LL29. TGFB receptor 1 and SMAD2 were identified as the targets of miR-27b by 3'-untranslated region luciferase reporter and western blotting assays. | |
dc.description.abstract | Conclusions: Our results suggest that miR-27b is an anti-fibrotic microRNA that inhibits fibroblast activation by targeting TGFB receptor 1 and SMAD2. This discovery may provide new targets for therapeutic interventions of idiopathic pulmonary fibrosis. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | BioMed Central | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | miR-27b inhibits fibroblast activation via targeting TGFB signaling pathway | |
osu.filename | oksd_zeng_mir-27binhibits_2017.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1186/s12860-016-0123-7 | |
dc.description.department | Physiological Sciences | |
dc.description.department | Oklahoma Center for Respiratory and Infectious Diseases | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | miR-27b | |
dc.subject.keywords | fibroblast activation | |
dc.subject.keywords | TGFB | |
dc.subject.keywords | idiopathic pulmonary fibrosis | |