dc.contributor.author | Schneider, Willian Daniel Hahn | |
dc.contributor.author | Goncalves, Thiago Augusto | |
dc.contributor.author | Uchima, Cristiane Akemi | |
dc.contributor.author | Couger, Matthew Brian | |
dc.contributor.author | Prade, Rolf | |
dc.contributor.author | Squina, Fabio Marcio | |
dc.contributor.author | Dillon, Aldo Jose Pinheiro | |
dc.contributor.author | Camassola, Marli | |
dc.date.accessioned | 2018-11-09T21:10:54Z | |
dc.date.available | 2018-11-09T21:10:54Z | |
dc.date.issued | 2016-03-17 | |
dc.identifier | oksd_schneider_penicilliumechi_2016 | |
dc.identifier.citation | Schneider, W. D. H., Goncalves, T. A., Uchima, C. A., Couger, M. B., Prade, R., Squina, F. M., ... Camassola, M. (2016). Penicillium echinulatum secretome analysis reveals the fungi potential for degradation of lignocellulosic biomass. Biotechnology for Biofuels, 9, Article 66. https://doi.org/10.1186/s13068-016-0476-3 | |
dc.identifier.uri | https://hdl.handle.net/11244/302070 | |
dc.description.abstract | Background: The enzymatic degradation of lignocellulosic materials by fungal enzyme systems has been extensively studied due to its effectiveness in the liberation of fermentable sugars for bioethanol production. Recently, variants of the fungus Penicillium echinulatum have been described as a great producer of cellulases and considered a promising strain for the bioethanol industry. | |
dc.description.abstract | Results: Penicillium echinulatum, wild-type 2HH and its mutant strain S1M29, were grown on four different carbon sources: cellulose, sugar cane bagasse pretreated by steam explosion (SCB), glucose, and glycerol for 120 h. Samples collected at 24, 96, and 120 h were used for enzymatic measurement, and the 96-h one was also used for secretome analysis by 1D-PAGE LC - MS/MS. A total of 165 proteins were identified, and more than one-third of these proteins belong to CAZy families. Glycosyl hydrolases (GH) are the most abundant group, being represented in larger quantities by GH3, 5, 17, 43, and 72. Cellobiohydrolases, endoglucanases, B-glycosidases, xylanases, B-xylosidases, and mannanases were found, and in minor quantities, pectinases, ligninases, and amylases were also found. Swollenin and esterases were also identified. | |
dc.description.abstract | Conclusions: Our study revealed differences in the two strains of P. echinulatum in several aspects in which the mutation improved the production of enzymes related to lignocellulosic biomass deconstruction. Considering the spectral counting analysis, the mutant strain S1M29 was more efficient in the production of enzymes involved in cellulose and hemicellulose degradation, despite having a nearly identical CAZy enzymatic repertoire. Moreover, S1M29 secretes more quantities of protein on SCB than on cellulose, relevant information when considering the production of cellulases using raw materials at low cost. Glucose, and especially glycerol, were used mainly for the production of amylases and ligninases. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | BioMed Central | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Penicillium echinulatum secretome analysis reveals the fungi potential for degradation of lignocellulosic biomass | |
osu.filename | oksd_schneider_penicilliumechi_2016.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1186/s13068-016-0476-3 | |
dc.description.department | Microbiology and Molecular Genetics | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | lignocellulosic biomass | |
dc.subject.keywords | biofuels | |
dc.subject.keywords | penicillium echinulatum strains | |
dc.subject.keywords | secretome | |
dc.subject.keywords | cazymes | |