dc.contributor.author | Joshi, Bharat D. | |
dc.contributor.author | Berg, Michael | |
dc.contributor.author | Rogers, Janet | |
dc.contributor.author | Fletcher, Jacqueline | |
dc.contributor.author | Melcher, Ulrich | |
dc.date.accessioned | 2018-11-09T21:10:49Z | |
dc.date.available | 2018-11-09T21:10:49Z | |
dc.date.issued | 2005-12-07 | |
dc.identifier | oksd_bharatdjoshi_sequencecompari_2005 | |
dc.identifier.citation | Joshi, B. D., Berg, M., Rogers, J., Fletcher, J., & Melcher, U. (2005). Sequence comparisons of plasmids pBJS-O of Spiroplasma citri and pSKU146 of S. kunkelii: Implications for plasmid evolution. BMC Genomics, 6, Article 175. https://doi.org/10.1186/1471-2164-6-175 | |
dc.identifier.uri | https://hdl.handle.net/11244/302061 | |
dc.description.abstract | Background: Spiroplasma citri BR3-3X and S. kunkelii CR2-3X cause serious diseases worldwide on citrus and maize species, respectively. S. citri BR3-3X harbors a plasmid, pBJS-Original (pBJS-O), that encodes the s piroplasma a dhesion r elated p rotein 1 (SARP1), a protein implicated in binding of the pathogen to cells of its leafhopper vector, Circulifer tenellus. The S. kunkelii CR2-3X plasmid, pSKU146, encodes a homolog of SARP1, Sk-ARP1. Due to the close phylogenetic relationship of the two pathogens, we hypothesized that the two plasmids are closely related as well. | |
dc.description.abstract | Results: The nucleotide sequence of pBJS-O was determined and compared to the sequences of a plasmid from BR3-T (pBJS-T), which is a multiply passaged leafhopper transmissible derivative of BR3-3X, and to known plasmid sequences including that of pSKU146. In addition to arp1, the 13,374 bp pBJS-O sequence putatively contains nine genes, recognized as open reading frames (ORFs). Several pBJS-O ORFs have homologs on pSKU146. However, the sequences flanking soj-like genes on both plasmids were found to be more distant from one another than sequences in any other region. Further, unlike pSKU146, pBJS-O lacks the conserved oriT region characteristic of the IncP group of bacterial plasmids. We were unable to identify a region in pBJS-O resembling a known plasmid origin of transfer. In regions where sequence was available for the plasmid from both BR3-3X and BR3-T, the pBJS-T sequence had a 0.4 kb deletion relative to its progenitor, pBJS-O. Southern blot hybridization of extrachromosomal DNA from various S. citri strains and spiroplasma species to an arp-specific probe and a probe made from the entire plasmid DNA of BR3-3X revealed limited conservation of both sequences in the genus Spiroplasma. Finally, we also report the presence on the BR3-3X chromosome of arp2, an S. citri homolog of arp1 that encodes the predicted protein SARP2. The C-terminal domain of SARP2 is homologous to that of SARP1, but its N-terminal domain is distinct. | |
dc.description.abstract | Conclusion: Our data suggest that pBJS is a novel S. citri plasmid that does not belong to any known plasmid incompatibility group. The differences between pBJS-O and pSKU146 suggest that one or more events of recombination have contributed to the divergence of the plasmids of the two sister Spiroplasma species; the plasmid from S. citri itself has diverged slightly during the derivation of S. citri BR3-T from BR3-3X. Our data also show that pBJS-O encodes the putative adhesin SARP1. The presence of traE and mob on pBJS-O suggests a role for the plasmid in spiroplasmal conjugation. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | BioMed Central | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Sequence comparisons of plasmids pBJS-O of Spiroplasma citri and pSKU146 of S. kunkelii: Implications for plasmid evolution | |
osu.filename | oksd_bharatdjoshi_sequencecompari_2005.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1186/1471-2164-6-175 | |
dc.description.department | Biochemistry and Molecular Biology | |
dc.description.department | Entomology and Plant Pathology | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | transmembrane helix | |
dc.subject.keywords | plasmid sequence | |
dc.subject.keywords | EMBL nucleotide sequence database | |
dc.subject.keywords | leafhopper vector | |
dc.subject.keywords | beet leafhopper | |