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dc.contributor.authorJagadeeswaran, Guru
dc.contributor.authorNimmakayala, Padma
dc.contributor.authorZheng, Yun
dc.contributor.authorGowdu, Kanchana
dc.contributor.authorReddy, Umesh K.
dc.contributor.authorSunkar, Ramanjulu
dc.date.accessioned2018-11-09T21:10:44Z
dc.date.available2018-11-09T21:10:44Z
dc.date.issued2012-07-23
dc.identifieroksd_jagadeeswaran_characterizatio_2012
dc.identifier.citationJagadeeswaran, G., Nimmakayala, P., Zheng, Y., Gowdu, K., Reddy, U. K., & Sunkar, R. (2012). Characterization of the small RNA component of leaves and fruits from four different cucurbit species. BMC Genomics, 13, Article 329. https://doi.org/10.1186/1471-2164-13-329
dc.identifier.urihttps://hdl.handle.net/11244/302049
dc.description.abstractBackground: MicroRNAs (miRNAs) are a class of non-coding small RNAs involved in post-transcriptional regulation of gene expression critical for plant growth and development, stress responses and other diverse biological processes in plants. The Cucurbitaceae or cucurbit family represents some of economically important species, particularly those with edible and medicinal fruits. Genomic tools for the molecular analysis of members of this family are just emerging. Partial draft genome sequence became available recently for cucumber and watermelon facilitating investigation of the small RNA component of the transcriptomes in cucurbits.
dc.description.abstractResults: We generated four small RNA libraries from bottle gourd (Lagenaria siceraria), Cucurbita moschata, Cucurbita pepo, and, watermelon (Citrullus lanatus var. lanatus) in order to identify conserved and novel lineage specific miRNAs in these cucurbits. Deep sequencing of small RNA libraries from these species resulted in 1,597,263, 532,948, 601,388, and 493,384 unique sRNA reads from bottle gourd, moschata, pepo and watermelon, respectively. Sequence analysis of these four libraries resulted in identification of 21 miRNA families that are highly conserved and 8 miRNA families that are moderately conserved in diverse dicots. We also identified 4 putative novel miRNAs in these plant species. Furthermore, the tasiRNAs were identified and their biogenesis was determined in these cucurbits. Small RNA blot analysis or q-PCR analyses of leaf and fruit tissues of these cucurbits showed differential expression of several conserved miRNAs. Interestingly, the abundance of several miRNAs in leaves and fruits of closely related C. moschata and C. pepo was also distinctly different. Target genes for the most conserved miRNAs are also predicted.
dc.description.abstractConclusion: High-throughput sequencing of small RNA libraries from four cucurbit species has provided a glimpse of small RNA component in their transcriptomes. The analysis also showed considerable variation within four cucurbit species with regards to expression of individual miRNAs.
dc.formatapplication/pdf
dc.languageen_US
dc.publisherBioMed Central
dc.rightsThis material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information.
dc.titleCharacterization of the small RNA component of leaves and fruits from four different cucurbit species
osu.filenameoksd_jagadeeswaran_characterizatio_2012.pdf
dc.description.peerreviewPeer reviewed
dc.identifier.doi10.1186/1471-2164-13-329
dc.description.departmentBiochemistry and Molecular Biology
dc.type.genreArticle
dc.type.materialText
dc.subject.keywordsmiRNA family
dc.subject.keywordsfruit tissue
dc.subject.keywordscucurbita pepo
dc.subject.keywordsbottle gourd
dc.subject.keywordsconserve miRNA family


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