dc.contributor.author | de la Fuente, Jose | |
dc.contributor.author | Maritz-Olivier, Christine | |
dc.contributor.author | Naranjo, Victoria | |
dc.contributor.author | Ayoubi, Patricia | |
dc.contributor.author | Nijhof, Ard M. | |
dc.contributor.author | Almazan, Consuelo | |
dc.contributor.author | Canales, Mario | |
dc.contributor.author | Perez de la Lastra, Jose M. | |
dc.contributor.author | Galindo, Ruth C. | |
dc.contributor.author | Blouin, Edmour F. | |
dc.contributor.author | Gortazar, Christian | |
dc.contributor.author | Jongejan, Frans | |
dc.contributor.author | Kocan, Katherine M. | |
dc.date.accessioned | 2018-11-09T21:10:41Z | |
dc.date.available | 2018-11-09T21:10:41Z | |
dc.date.issued | 2008-08-02 | |
dc.identifier | oksd_delafuente_evidenceofthero_2008 | |
dc.identifier.citation | de la Fuente, J., Maritz-Olivier, C., Naranjo, V., Ayoubi, P., Nijhof, A. M., Almazan, C., ... Kocan, K. M. (2008). Evidence of the role of tick subolesin in gene expression. BMC Genomics, 9, Article 372. https://doi.org/10.1186/1471-2164-9-372 | |
dc.identifier.uri | https://hdl.handle.net/11244/302042 | |
dc.description.abstract | Background: Subolesin is an evolutionary conserved protein that was discovered recently in Ixodes scapularis as a tick protective antigen and has a role in tick blood digestion, reproduction and development. In other organisms, subolesin orthologs may be involved in the control of developmental processes. Because of the profound effect of subolesin knockdown in ticks and other organisms, we hypothesized that subolesin plays a role in gene expression, and therefore affects multiple cellular processes. The objective of this study was to provide evidence for the role of subolesin in gene expression. | |
dc.description.abstract | Results: Two subolesin-interacting proteins were identified and characterized by yeast two-hybrid screen, co-affinity purification and RNA interference (RNAi). The effect of subolesin knockdown on the tick gene expression pattern was characterized by microarray analysis and demonstrated that subolesin RNAi affects the expression of genes involved in multiple cellular pathways. The analysis of subolesin and interacting protein sequences identified regulatory motifs and predicted the presence of conserved protein kinase C (PKC) phosphorylation sites. | |
dc.description.abstract | Conclusion: Collectively, these results provide evidence that subolesin plays a role in gene expression in ticks. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | BioMed Central | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Evidence of the role of tick subolesin in gene expression | |
osu.filename | oksd_delafuente_evidenceofthero_2008.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1186/1471-2164-9-372 | |
dc.description.department | Veterinary Pathobiology | |
dc.description.department | Biochemistry and Molecular Biology | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | tick Infestation | |
dc.subject.keywords | female tick | |
dc.subject.keywords | control tick | |
dc.subject.keywords | injection buffer | |
dc.subject.keywords | synthetic define | |