dc.contributor.author | Booth, Melissa G. | |
dc.contributor.author | Hutchinson, LeAnna | |
dc.contributor.author | Brumsted, Margaret | |
dc.contributor.author | Aas, Peter | |
dc.contributor.author | Coffin, Richard B. | |
dc.contributor.author | Downer, Roswell C., Jr. | |
dc.contributor.author | Kelley, Cheryl A. | |
dc.contributor.author | Lyons, M. Maille | |
dc.contributor.author | Pakulski, J. Dean | |
dc.contributor.author | Holder Sandvik, Sonya L. | |
dc.contributor.author | Jeffrey, Wade H. | |
dc.contributor.author | Miller, Robert V. | |
dc.date.accessioned | 2018-09-21T17:51:41Z | |
dc.date.available | 2018-09-21T17:51:41Z | |
dc.date.issued | 2001-03-28 | |
dc.identifier | oksd_booth_quantificationo_2001 | |
dc.identifier.citation | Booth, M. G., Hutchinson, L., Brumsted, M., Aas, P., Coffin, R. B., Downer, R. C., Jr., ... Miller, R. V. (2001). Quantification of recA gene expression as an indicator of repair potential in marine bacterioplankton communities of Antarctica. Aquatic Microbial Ecology, 24(1), 51-59. https://doi.org/10.3354/ame024051 | |
dc.identifier.uri | https://hdl.handle.net/11244/301781 | |
dc.description.abstract | Marine bacteria in surface waters must cope daily with the damaging effects of exposure to solar radiation (containing both UV-A and UV-B wavelengths), which produces lesions in their DNA. As the stratospheric ozone layer is depleted, these coping mechanisms are likely to play an even more important role in the viability of marine bacterial communities. The recA gene is ubiquitous among eubacteria and is highly conserved both in nucleotide and amino acid sequence. Besides its role in generalized recombination, the gene's translational product, RecA, is the regulator of 'dark repair' activity (DNA-repair mechanisms that do not require visible light as a cofactor). We have taken advantage of this function and used recA gene expression as a barometer of the DNAdamage repair capacity of bacterial assemblages in the Southern Ocean. Studies were conducted in the Gerlache Strait, Antarctica, in the austral springs of 1995 and 1996. Analysis of both recA mRNA and RecA protein extracted from natural communities indicated that the level of expression of this gene varied in a diel fashion, suggesting an increased repair capacity in these organisms. These included an early morning rise in RecA levels followed by a plateau or even a reduction in RecA concentration during the remainder of the day. A much greater increase in RecA was consistently observed after sunset, followed by a constant decrease during the night. Microcosm experiments with a RecA+ Gerlache Strait g-proteobacteria isolate, RM11001, demonstrated a similar diel pattern of expression. These studies demonstrate the usefulness of RecA as a biological indicator of DNA repair capacity in natural bacterial assemblages. They indicate that 'dark repair' of DNA damage is an important coping mechanism for bacteria in the marine environment of Antarctica. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | Inter Research | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Quantification of recA gene expression as an indicator of repair potential in marine bacterioplankton communities of Antarctica | |
osu.filename | oksd_booth_quantificationo_2001.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.3354/ame024051 | |
dc.description.department | Microbiology and Molecular Genetics | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | southern ocean | |
dc.subject.keywords | antarctica | |
dc.subject.keywords | solar uv radiation | |
dc.subject.keywords | dna repair | |
dc.subject.keywords | marine bacterioplankton communities | |
dc.subject.keywords | reca gene | |
dc.subject.keywords | reca protein | |