dc.contributor.author | Youssef, Noha H. | |
dc.contributor.author | Couger, M. B. | |
dc.contributor.author | Struchtemeyer, Christopher G. | |
dc.contributor.author | Liggenstoffer, Audra S. | |
dc.contributor.author | Prade, Rolf A. | |
dc.contributor.author | Najar, Fares Z. | |
dc.contributor.author | Atiyeh, Hasan K. | |
dc.contributor.author | Wilkins, Mark R. | |
dc.contributor.author | Elshahed, Mostafa S. | |
dc.date.accessioned | 2018-09-21T17:51:39Z | |
dc.date.available | 2018-09-21T17:51:39Z | |
dc.date.issued | 2013-08 | |
dc.identifier | oksd_youssef_thegenomeofthea_2013 | |
dc.identifier.citation | Youssef, N. H., Couger, M. B., Struchtemeyer, C. G., Liggenstoffer, A. S., Prade, R. A., Najar, F. Z., ... Elshahed, M. S. (2013). The genome of the anaerobic fungus Orpinomyces sp. strain C1A reveals the unique evolutionary history of a remarkable plant biomass degrader. Applied and Environmental Microbiology, 79(15), 4620-4634. https://doi.org/10.1128/AEM.00821-13 | |
dc.identifier.uri | https://hdl.handle.net/11244/301776 | |
dc.description.abstract | Anaerobic gut fungi represent a distinct early-branching fungal phylum (Neocallimastigomycota) and reside in the rumen, hindgut, and feces of ruminant and nonruminant herbivores. The genome of an anaerobic fungal isolate, Orpinomyces sp. strain C1A, was sequenced using a combination of Illumina and PacBio single-molecule real-time (SMRT) technologies. The large genome (100.95 Mb, 16,347 genes) displayed extremely low G+C content (17.0%), large noncoding intergenic regions (73.1%), proliferation of microsatellite repeats (4.9%), and multiple gene duplications. Comparative genomic analysis identified multiple genes and pathways that are absent in Dikarya genomes but present in early-branching fungal lineages and/or nonfungal Opisthokonta. These included genes for posttranslational fucosylation, the production of specific intramembrane proteases and extracellular protease inhibitors, the formation of a complete axoneme and intraflagellar trafficking machinery, and a near-complete focal adhesion machinery. Analysis of the lignocellulolytic machinery in the C1A genome revealed an extremely rich repertoire, with evidence of horizontal gene acquisition from multiple bacterial lineages. Experimental analysis indicated that strain C1A is a remarkable biomass degrader, capable of simultaneous saccharification and fermentation of the cellulosic and hemicellulosic fractions in multiple untreated grasses and crop residues examined, with the process significantly enhanced by mild pretreatments. This capability, acquired during its separate evolutionary trajectory in the rumen, along with its resilience and invasiveness compared to prokaryotic anaerobes, renders anaerobic fungi promising agents for consolidated bioprocessing schemes in biofuels production. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | American Society for Microbiology | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Genome of the anaerobic fungus Orpinomyces sp. strain C1A reveals the unique evolutionary history of a remarkable plant biomass degrader | |
osu.filename | oksd_youssef_thegenomeofthea_2013.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1128/AEM.00821-13 | |
dc.description.department | Microbiology and Molecular Genetics | |
dc.description.department | Biosystems and Agricultural Engineering | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | adaptation, physiological | |
dc.subject.keywords | animals | |
dc.subject.keywords | biomass | |
dc.subject.keywords | cattle | |
dc.subject.keywords | cellulose | |
dc.subject.keywords | evolution, molecular | |
dc.subject.keywords | feces | |
dc.subject.keywords | fermentation | |
dc.subject.keywords | genome, fungal | |
dc.subject.keywords | molecular sequence data | |
dc.subject.keywords | neocallimastigales | |
dc.subject.keywords | phylogeny | |