dc.contributor.author | Lu, Jingrang | |
dc.contributor.author | Ryu, Hodon | |
dc.contributor.author | Vogel, Jason | |
dc.contributor.author | Santo Domingo, Jorge | |
dc.contributor.author | Ashbolt, Nicholas J. | |
dc.date.accessioned | 2018-09-21T17:51:29Z | |
dc.date.available | 2018-09-21T17:51:29Z | |
dc.date.issued | 2013-06 | |
dc.identifier | oksd_lu_moleculardetect_2013 | |
dc.identifier.citation | Lu, J., Ryu, H., Vogel, J., Santo Domingo, J., & Ashbolt, N. J. (2013). Molecular detection of Campylobacter spp. and fecal indicator bacteria during the northern migration of sandhill cranes (Grus canadensis) at the central Platte River. Applied and Environmental Microbiology, 79(12), 3762-3769. https://doi.org/10.1128/AEM.03990-12 | |
dc.identifier.uri | https://hdl.handle.net/11244/301750 | |
dc.description.abstract | The risk to human health of the annual sandhill crane (Grus canadensis) migration through Nebraska, which is thought to be a major source of fecal pollution of the central Platte River, is unknown. To better understand potential risks, the presence of Campylobacter species and three fecal indicator bacterial groups (Enterococcus spp., Escherichia coli, and Bacteroidetes) was assayed by PCR from crane excreta and water samples collected during their stopover at the Platte River, Nebraska, in 2010. Genus-specific PCR assays and sequence analyses identified Campylobacter jejuni as the predominant Campylobacter species in sandhill crane excreta. Campylobacter spp. were detected in 48% of crane excreta, 24% of water samples, and 11% of sediment samples. The estimated densities of Enterococcus spp. were highest in excreta samples (mean, 4.6 x 10^8 cell equivalents [CE]/g), while water samples contained higher levels of Bacteroidetes (mean, 5.1 x 10^5 CE/100 ml). Enterococcus spp., E. coli, and Campylobacter spp. were significantly increased in river water and sediments during the crane migration period, with Enterococcus sp. densities (~3.3 x 10^5 CE/g) 2 to 4 orders of magnitude higher than those of Bacteroidetes (4.9 x 10^3 CE/g), E. coli (2.2 x 10^3 CE/g), and Campylobacter spp. (37 CE/g). Sequencing data for the 16S rRNA gene and Campylobacter species-specific PCR assays indicated that C. jejuni was the major Campylobacter species present in water, sediments, and crane excreta. Overall, migration appeared to result in a significant, but temporary, change in water quality in spring, when there may be a C. jejuni health hazard associated with water and crops visited by the migrating birds. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | American Society for Microbiology | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Molecular detection of Campylobacter spp. and fecal indicator bacteria during the northern migration of sandhill cranes (Grus canadensis) at the central Platte River | |
osu.filename | oksd_lu_moleculardetect_2013.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1128/AEM.03990-12 | |
dc.description.department | Biosystems and Agricultural Engineering | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | animal migration | |
dc.subject.keywords | animals | |
dc.subject.keywords | bacteroidetes | |
dc.subject.keywords | base sequence | |
dc.subject.keywords | bird diseases | |
dc.subject.keywords | birds | |
dc.subject.keywords | campylobacter | |
dc.subject.keywords | enterococcus | |
dc.subject.keywords | escherichia coli | |
dc.subject.keywords | feces | |
dc.subject.keywords | molecular sequence data | |
dc.subject.keywords | nebraska | |
dc.subject.keywords | polymerase chain reaction | |
dc.subject.keywords | population dynamics | |
dc.subject.keywords | soil microbiology | |
dc.subject.keywords | water microbiology | |
dc.subject.keywords | water quality | |