dc.contributor.author | Dalvi, Sonal | |
dc.contributor.author | Azetsu, Sei | |
dc.contributor.author | Patrauchan, Marianna A. | |
dc.contributor.author | Aktas, Deniz F. | |
dc.contributor.author | Fathepure, Babu Z. | |
dc.date.accessioned | 2018-09-21T17:51:23Z | |
dc.date.available | 2018-09-21T17:51:23Z | |
dc.date.issued | 2012-10 | |
dc.identifier | oksd_dalvi_proteogenomice_2012 | |
dc.identifier.citation | Dalvi, S., Azetsu, S., Patrauchan, M. A., Aktas, D. F., & Fathepure, B. Z. (2012). Proteogenomic elucidation of the initial steps in the benzene degradation pathway of a novel halophile, Arhodomonas sp. strain Rozel, isolated from a hypersaline environment. Applied and Environmental Microbiology, 78(20), 7309-7316. https://doi.org/10.1128/AEM.01327-12 | |
dc.identifier.uri | https://hdl.handle.net/11244/301736 | |
dc.description.abstract | Lately, there has been a special interest in understanding the role of halophilic and halotolerant organisms for their ability to degrade hydrocarbons. The focus of this study was to investigate the genes and enzymes involved in the initial steps of the benzene degradation pathway in halophiles. The extremely halophilic bacteria Arhodomonas sp. strain Seminole and Arhodomonas sp. strain Rozel, which degrade benzene and toluene as the sole carbon source at high salinity (0.5 to 4 M NaCl), were isolated from enrichments developed from contaminated hypersaline environments. To obtain insights into the physiology of this novel group of organisms, a draft genome sequence of the Seminole strain was obtained. A cluster of 13 genes predicted to be functional in the hydrocarbon degradation pathway was identified from the sequence. Two-dimensional (2D) gel electrophoresis and liquid chromatography-mass spectrometry were used to corroborate the role of the predicted open reading frames (ORFs). ORFs 1080 and 1082 were identified as components of a multicomponent phenol hydroxylase complex, and ORF 1086 was identified as catechol 2,3-dioxygenase (2,3-CAT). Based on this analysis, it was hypothesized that benzene is converted to phenol and then to catechol by phenol hydroxylase components. The resulting catechol undergoes ring cleavage via the meta pathway by 2,3-CAT to form 2-hydroxymuconic semialdehyde, which enters the tricarboxylic acid cycle. To substantiate these findings, the Rozel strain was grown on deuterated benzene, and gas chromatography-mass spectrometry detected deuterated phenol as the initial intermediate of benzene degradation. These studies establish the initial steps of the benzene degradation pathway in halophiles. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | American Society for Microbiology | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Proteogenomic elucidation of the initial steps in the benzene degradation pathway of a novel halophile, Arhodomonas sp. strain Rozel, isolated from a hypersaline environment | |
osu.filename | oksd_dalvi_proteogenomice_2012.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1128/AEM.01327-12 | |
dc.description.department | Microbiology and Molecular Genetics | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | benzene | |
dc.subject.keywords | carbon | |
dc.subject.keywords | chromatography, liquid | |
dc.subject.keywords | dna, bacterial | |
dc.subject.keywords | ectothiorhodospiraceae | |
dc.subject.keywords | electrophoresis, gel, two-dimensional | |
dc.subject.keywords | environmental microbiology | |
dc.subject.keywords | genome, bacterial | |
dc.subject.keywords | mass spectrometry | |
dc.subject.keywords | metabolic networks and pathways | |
dc.subject.keywords | molecular sequence data | |
dc.subject.keywords | multigene family | |
dc.subject.keywords | phenol | |
dc.subject.keywords | salinity | |
dc.subject.keywords | sequence analysis, dna | |
dc.subject.keywords | toluene | |
dc.subject.keywords | dna, bacterial | |
dc.subject.keywords | phenol | |
dc.subject.keywords | toluene | |
dc.subject.keywords | carbon | |
dc.subject.keywords | benzene | |