dc.contributor.author | Dalvi, Sonal | |
dc.contributor.author | Nicholson, Carla | |
dc.contributor.author | Najar, Fares | |
dc.contributor.author | Roe, Bruce A. | |
dc.contributor.author | Canaan, Patricia | |
dc.contributor.author | Hartson, Steven D. | |
dc.contributor.author | Fathepure, Babu Z. | |
dc.date.accessioned | 2018-09-21T17:51:23Z | |
dc.date.available | 2018-09-21T17:51:23Z | |
dc.date.issued | 2014-11 | |
dc.identifier | oksd_dalvi_arhodomonassp.s_2014 | |
dc.identifier.citation | Dalvi, S., Nicholson, C., Najar, F., Roe, B. A., Canaan, P., Hartson, S. D., & Fathepure, B. Z. (2014). Arhodomonas sp. strain Seminole and its genetic potential to degrade aromatic compounds under high-salinity conditions. Applied and Environmental Microbiology, 80(21), 6664-6676. https://doi.org/10.1128/AEM.01509-14 | |
dc.identifier.uri | https://hdl.handle.net/11244/301735 | |
dc.description.abstract | Arhodomonas sp. strain Seminole was isolated from a crude oil-impacted brine soil and shown to degrade benzene, toluene, phenol, 4-hydroxybenzoic acid (4-HBA), protocatechuic acid (PCA), and phenylacetic acid (PAA) as the sole sources of carbon at high salinity. Seminole is a member of the genus Arhodomonas in the class Gammaproteobacteria, sharing 96% 16S rRNA gene sequence similarity with Arhodomonas aquaeolei HA-1. Analysis of the genome predicted a number of catabolic genes for the metabolism of benzene, toluene, 4-HBA, and PAA. The predicted pathways were corroborated by identification of enzymes present in the cytosolic proteomes of cells grown on aromatic compounds using liquid chromatography-mass spectrometry. Genome analysis predicted a cluster of 19 genes necessary for the breakdown of benzene or toluene to acetyl coenzyme A (acetyl-CoA) and pyruvate. Of these, 12 enzymes were identified in the proteome of toluene-grown cells compared to lactate-grown cells. Genomic analysis predicted 11 genes required for 4-HBA degradation to form the tricarboxylic acid (TCA) cycle intermediates. Of these, proteomic analysis of 4-HBA-grown cells identified 6 key enzymes involved in the 4-HBA degradation pathway. Similarly, 15 genes needed for the degradation of PAA to the TCA cycle intermediates were predicted. Of these, 9 enzymes of the PAA degradation pathway were identified only in PAA-grown cells and not in lactate-grown cells. Overall, we were able to reconstruct catabolic steps for the breakdown of a variety of aromatic compounds in an extreme halophile, strain Seminole. Such knowledge is important for understanding the role of Arhodomonas spp. in the natural attenuation of hydrocarbon-impacted hypersaline environments. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | American Society for Microbiology | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Arhodomonas sp. strain Seminole and its genetic potential to degrade aromatic compounds under high-salinity conditions | |
osu.filename | oksd_dalvi_arhodomonassp.s_2014.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1128/AEM.01509-14 | |
dc.description.department | Microbiology and Molecular Genetics | |
dc.description.department | Biochemistry and Molecular Biology | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | cells | |
dc.subject.keywords | phenylacetates | |
dc.subject.keywords | pyruvates | |
dc.subject.keywords | acids | |
dc.subject.keywords | soil | |
dc.subject.keywords | phenols | |
dc.subject.keywords | parabens | |
dc.subject.keywords | benzene | |
dc.subject.keywords | hydrocarbons | |
dc.subject.keywords | acetyl coenzyme a | |
dc.subject.keywords | petroleum | |
dc.subject.keywords | carbon | |
dc.subject.keywords | lactates | |
dc.subject.keywords | enzymes | |
dc.subject.keywords | salinity | |
dc.subject.keywords | citric acid cycle | |
dc.subject.keywords | environment | |
dc.subject.keywords | metabolism | |
dc.subject.keywords | genes | |