Loading...
Thumbnail Image

Date

2018-07-17

Journal Title

Journal ISSN

Volume Title

Publisher

Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International

Kinetic doping has previously been shown to be an effective method of doping silica sol−gel thin films with an enzyme to construct biosensors. Until now, kinetic doping has only been applied to films produced through the spincoating method. In this study, we present the use of dipcoating to produce thin films kinetically doped for biosensor development. In this way, kinetically doped biosensors may benefit from the increased range of substrate material shapes and sizes that may be easily coated through dip-coating but not spin-coating. The biosensors produced through dipcoating continue to show enhanced performance over more conventional enzyme loading methods with horseradish peroxidase and cytochrome C samples, showing an increase of 2400× and 1300× in enzyme concentration over that in their loading solutions, respectively. These correspond to enzyme concentrations of 5.37 and 10.57 mmol/L all while preserving a modest catalytic activity for the detection of hydrogen peroxide by horseradish peroxidase. This leads to a 77% and 88% increase in the total amount of horseradish peroxidase and cytochrome C, respectively, over that from coating the same glass coverslip via spincoating methods.

Description

Keywords

Chemistry, Biochemistry., Silica sol-gel, Kinetic doping, Thin film biosensors, Horseradish Peroxidase, Hydrogen Peroxide sensor

Citation

Kinetically Doped Silica Sol–Gel Optical Biosensors: Expanding Potential Through Dip-Coating Matthew S. Crosley and Wai Tak Yip ACS Omega 2018 3 (7), 7971-7978 DOI: 10.1021/acsomega.8b00897

Related file

Notes

Sponsorship

This work was supported by Oklahoma Centre for the Advancement of Science and Technology (HR 12-128). Article processing charges funded in part by University of Oklahoma Libraries.