dc.contributor.author | Sanborn, M. R. | |
dc.contributor.author | Wan, S. K. | |
dc.contributor.author | Bulard, R. | |
dc.date.accessioned | 2018-08-17T16:24:55Z | |
dc.date.available | 2018-08-17T16:24:55Z | |
dc.date.issued | 1982-10 | |
dc.identifier | oksd_mrsanborn_microwavesteril_1982 | |
dc.identifier.citation | Sanborn, M. R., Wan, S. K., & Bulard, R. (1982). Microwave sterilization of plastic tissue culture vessels for reuse. Applied and Environmental Microbiology, 44(4), 960-964. https://doi.org/10.1128/aem.44.4.960-964.1982 | |
dc.identifier.uri | https://hdl.handle.net/11244/301447 | |
dc.description.abstract | A simple protocol has been developed for recycling plastic tissue culture vessels. The killing properties of microwaves were used to decontaminate plastic tissue culture vessels for reuse. Nine bacterial cultures, four gram-negative and five gram-positive genera, including two Bacillus species, were used to artificially contaminate tissue culture vessels. The microwaves produced by a "home-type" microwave oven (2.45 gHz) were able to decontaminate the vessels with a 3-min exposure. The same exposure time was also used to completely inactivate the following three test viruses: polio type 1, parainfluenza type 1 (Sendai), and bacteriophage T4. The recycling procedure did not reduce the attachment and proliferation of the following cell types: primary chicken and turkey embryo, HEp-2, Vero, BGMK, and MK-2. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | American Society for Microbiology | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Microwave sterilization of plastic tissue culture vessels for reuse | |
osu.filename | oksd_mrsanborn_microwavesteril_1982.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1128/aem.44.4.960-964.1982 | |
dc.description.department | Microbiology | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | bacteria | |
dc.subject.keywords | culture techniques | |
dc.subject.keywords | microwaves | |
dc.subject.keywords | sterilization | |
dc.subject.keywords | viruses | |