dc.contributor.author | Ayoubi, Patricia J. | |
dc.contributor.author | Harker, Alan R. | |
dc.date.accessioned | 2018-08-17T16:24:52Z | |
dc.date.available | 2018-08-17T16:24:52Z | |
dc.date.issued | 1998-11 | |
dc.identifier | oksd_patriciaj.ayoubi_whole-cellkinet_1998 | |
dc.identifier.citation | Ayoubi, P. J., & Harker, A. R. (1998). Whole-cell kinetics of trichloroethylene degradation by phenol hydroxylase in a Ralstonia eutropha JMP134 derivative. Applied and Environmental Microbiology, 64(11), 4353-4356. https://doi.org/10.1128/AEM.64.11.4353-4356.1998 | |
dc.identifier.uri | https://hdl.handle.net/11244/301439 | |
dc.description.abstract | The rate, progress, and limits of trichloroethylene (TCE) degradation by Ralstonia eutropha AEK301/pYK3021 whole cells were examined in the absence of aromatic induction. At TCE concentrations up to 800 uM, degradation rates were sustained until TCE was no longer detectable. The Ks and Vmax for TCE degradation by AEK301/pYK3021 whole cells were determined to be 630 uM and 22.6 nmol/min/mg of total protein, respectively. The sustained linear rates of TCE degradation by AEK301/pYK3021 up to a concentration of 800 uM TCE suggest that solvent effects are limited during the degradation of TCE and that this construct is little affected by the formation of toxic intermediates at the TCE levels and assay duration tested. TCE degradation by this strain is subject to carbon catabolite repression. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | American Society for Microbiology | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Whole-cell kinetics of trichloroethylene degradation by phenol hydroxylase in a Ralstonia eutropha JMP134 derivative | |
osu.filename | oksd_patriciaj.ayoubi_whole-cellkinet_1998.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1128/AEM.64.11.4353-4356.1998 | |
dc.description.department | Microbiology and Molecular Genetics | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | ralstonia eutropha | |
dc.subject.keywords | recombinant microorganism | |
dc.subject.keywords | ethylene(trichloro) | |
dc.subject.keywords | chlorocarbon | |
dc.subject.keywords | pollutant | |
dc.subject.keywords | biodegradation | |
dc.subject.keywords | kinetics | |
dc.subject.keywords | bioremediation | |
dc.subject.keywords | phenol 2-monooxygenase | |
dc.subject.keywords | bacteria | |
dc.subject.keywords | oxidoreductases | |
dc.subject.keywords | enzyme | |
dc.subject.keywords | microorganisme recombinant | |