dc.contributor.author | Filer, Keith | |
dc.contributor.author | Harker, Alan R. | |
dc.date.accessioned | 2018-08-17T16:24:52Z | |
dc.date.available | 2018-08-17T16:24:52Z | |
dc.date.issued | 1997-01 | |
dc.identifier | oksd_k.filer_identification_1997 | |
dc.identifier.citation | Filer, K., & Harker, A. R. (1997). Identification of the inducing agent of the 2,4-dichlorophenoxyacetic acid pathway encoded by plasmid pJP4. Applied and Environmental Microbiology, 63(1), 317-320. https://doi.org/10.1128/aem.63.1.317-320.1997 | |
dc.identifier.uri | https://hdl.handle.net/11244/301438 | |
dc.description.abstract | The inducing agent of the 2,4-dichlorophenoxyacetic acid (2,4-D) pathway of Alcaligenes eutrophus JMP134 (pJP4) was determined through the analysis of promoterless lacZ transcriptional fusions with tfd structural genes. (beta)-Galactosidase activity was measured in the presence and absence of 2,4-D. Fusions of the individual genes act both as reporters and disrupters of gene expression. Increases in reporter activity were expected in fusions occurring in genes which encode enzymes which function after the production of the inducing intermediate. This analysis indicates that dichloromuconate is the inducing intermediate. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | American Society for Microbiology | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Identification of the inducing agent of the 2,4-dichlorophenoxyacetic acid pathway encoded by plasmid pJP4 | |
osu.filename | oksd_k.filer_identification_1997.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1128/aem.63.1.317-320.1997 | |
dc.description.department | Microbiology and Molecular Genetics | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | ralstonia eutropha | |
dc.subject.keywords | chlorine organic compounds | |
dc.subject.keywords | biodegradation | |
dc.subject.keywords | metabolism | |
dc.subject.keywords | gene expression | |
dc.subject.keywords | plasmid | |
dc.subject.keywords | induction | |
dc.subject.keywords | hybrid gene | |
dc.subject.keywords | reporter gene | |
dc.subject.keywords | herbicide | |
dc.subject.keywords | bacteria | |
dc.subject.keywords | glycosidases | |
dc.subject.keywords | hydrolases | |
dc.subject.keywords | enzyme | |