dc.contributor.author | Sundin, G. W. | |
dc.contributor.author | Bender, C. L. | |
dc.date.accessioned | 2018-08-17T16:24:48Z | |
dc.date.available | 2018-08-17T16:24:48Z | |
dc.date.issued | 1993-04 | |
dc.identifier | oksd_gwsundin_ecologicalandge_1993 | |
dc.identifier.citation | Sundin, G. W. & Bender, C. L. (1993). Ecological and genetic analysis of copper and streptomycin resistance in Pseudomonas syringae pv. syringae. Applied and Environmental Microbiology, 59(4), 1018-1024. https://doi.org/10.1128/aem.59.4.1018-1024.1993 | |
dc.identifier.uri | https://hdl.handle.net/11244/301430 | |
dc.description.abstract | Strains of Pseudomonas syringae pv. syringae resistant to copper, streptomycin, or both compounds were recovered from symptomless and diseased tissue of four woody hosts in three nurseries in Oklahoma. In strains resistant to copper and streptomycin (Cu^r Sm^r), resistance to both compounds was cotransferred with a single plasmid which was either 68, 190, or 220 kilobase pairs (kb). All Cu^s Sm^r strains contained a 68-kb conjugative plasmid. Cu^r Sm^s, strains contained one plasmid which varied in size from 60 to 73 kb. All conjugative plasmids which transferred streptomycin resistance contained sequences homologous to the strA and strB Sm^r genes from the broad-host-range plasmid RSF1010. The Sm^r determinant was subsequently cloned from a 68-kb Cu^r Sm^r plasmid designated pPSR1. A restriction map detailing the organization of the homologous Sm^r genes from pPSR1 and RSF1010 and cloned Sm^r genes from P. syringae pv. papulans and Xanthomonas campestris pv. vesicatoria revealed the conservation of all sites studied. The Cu^r genes cloned from P. syringae pv. tomato PT23 and X. campestris pv. vesicatoria XV10 did not hybridize to the Cu^r plasmids identified in the present study, indicating that copper resistance in these P. syringae pv. syringae strains may be conferred by a distinct genetic determinant. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | American Society for Microbiology | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Ecological and genetic analysis of copper and streptomycin resistance in Pseudomonas syringae pv. syringae | |
osu.filename | oksd_gwsundin_ecologicalandge_1993.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1128/aem.59.4.1018-1024.1993 | |
dc.description.department | Plant Pathology | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | plasmid | |
dc.subject.keywords | characterization | |
dc.subject.keywords | resistance | |
dc.subject.keywords | molecular cloning | |
dc.subject.keywords | molecular hybridization | |
dc.subject.keywords | microorganism culture | |
dc.subject.keywords | gel electrophoresis | |
dc.subject.keywords | culture medium | |
dc.subject.keywords | xanthomonas campestris | |
dc.subject.keywords | pseudomonadaceae | |
dc.subject.keywords | pseudomonadales | |