Comparative Study of Some Dehydration and Clearing Agents
dc.contributor.advisor | Moore, Geo. A. | |
dc.contributor.author | Ralph, Paul | |
dc.date.accessioned | 2018-06-19T14:23:08Z | |
dc.date.available | 2018-06-19T14:23:08Z | |
dc.date.issued | 1937 | |
dc.identifier.uri | https://hdl.handle.net/11244/300211 | |
dc.description.abstract | An experiment to determine the advantages of dioxan, iso-butyl alcohol, tertiary butyl alcohol, and ethyl alcohol as dehydrants and chloroform, toluol, xylene, benzol, methyl benzoate, methyl salicylate, and acetone as clearers is described. Materials fixed in Bouin's fluid, Zenker formol, and IO% neutral formalin were dehydrated, embedded, sectioned, and stained. Bouin's fluid produces less hardening, shrinkage and distortion than the other fixatives employed. Slow dioxan is the best method of dehydration. All the picric acid need not be removed from tissues to be embedded in paraffin. Tissue blocks not more than 4 mm. thick may be dehydrated and impregnated with paraffin by slow dioxan in 13 hours, fast dioxan in 10 hours, iso-butyl alcohol and tertiary butyl alcohol in 14 hours, and ethyl alcohol-chloroform in 17 hours without incurring any distortion due to rapidity of dehydration and infiltration. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.rights | Copyright is held by the author who has granted the Oklahoma State University Library the non-exclusive right to share this material in its institutional repository. Contact Digital Library Services at lib-dls@okstate.edu or 405-744-9161 for the permission policy on the use, reproduction or distribution of this material. | |
dc.title | Comparative Study of Some Dehydration and Clearing Agents | |
osu.filename | Thesis-1937-R13c.pdf | |
osu.accesstype | Open Access | |
dc.description.department | Department of Integrative Biology | |
dc.type.genre | Thesis | |
dc.type.material | text |
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OSU Theses [15752]