dc.contributor.author | Xiaoping Gou | |
dc.contributor.author | Hongju Yin | |
dc.contributor.author | Kai He | |
dc.contributor.author | Junbo Du | |
dc.contributor.author | Jing Yi | |
dc.contributor.author | Shengbao Xu | |
dc.contributor.author | Honghui Lin | |
dc.contributor.author | Steven D. Clouse | |
dc.contributor.author | Jia Li | |
dc.date.accessioned | 2016-01-08T19:47:47Z | |
dc.date.accessioned | 2016-03-30T15:32:55Z | |
dc.date.available | 2016-01-08T19:47:47Z | |
dc.date.available | 2016-03-30T15:32:55Z | |
dc.date.issued | 2012-01-12 | |
dc.identifier.citation | Gou X, Yin H, He K, Du J, Yi J, Xu S, et al. (2012) Genetic Evidence for an Indispensable Role of Somatic Embryogenesis Receptor Kinases in Brassinosteroid Signaling. PLoS Genet 8(1): e1002452. doi:10.1371/journal.pgen.1002452 | en_US |
dc.identifier.uri | https://hdl.handle.net/11244/23531 | |
dc.description | The authors are grateful to the Arabidopsis Biological Resource Center for providing the T-DNA insertion lines discussed in this work. We thank Dr. Yanhai Yin (Iowa State University) for providing anti-BES1 antibody, Dr. Jiayang Li (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for bri1-301 seeds, and Dr. Xing-wang Deng (Yale University) for cop1-4 and cop1-6 seeds as controls. | en_US |
dc.description | | en_US |
dc.description.abstract | Author Summary Brassinosteroids (BRs) are a group of plant hormones critical for plant growth and development. BRs are perceived by a cell-surface receptor complex including two distinctive receptor kinases, BRI1 and BAK1. Whereas BRI1 is a true BR-binding receptor, BAK1 does not appear to have BR-binding activity. Therefore, BAK1 is likely a co-receptor in BR signal transduction. The genetic significance of BAK1 was not clearly demonstrated in previous studies largely due to functional redundancy of BAK1 and its closely related homologues. It was not clear whether BAK1 plays an essential role or only an enhancing role in BR signaling. In this study, we identified all possible BAK1 redundant genes in the Arabidopsis thaliana genome and generated single, double, triple, and quadruple mutants. Detailed analysis indicated that, without BAK1 and its functionally redundant proteins, BR signaling is completely disrupted, largely because BRI1 has lost its ability to activate downstream components. These studies provide the first piece of loss-of-functional genetic evidence that BAK1 is indispensable to the early events of the BR signaling pathway. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | PLos Genetics | |
dc.relation.ispartofseries | PLoS Genet 8(1):e1002452 | |
dc.relation.uri | http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1002452 | |
dc.rights | Attribution 3.0 United States | |
dc.rights.uri | https://creativecommons.org/licenses/by/3.0/us/ | |
dc.subject | Phenotypes,Phosphorylation,Seedlings,Hypocotyl,Hyperexpression techniques,Root growth,Genetically modified plants,Membrane proteins | en_US |
dc.title | Genetic Evidence for an Indispensable Role of Somatic Embryogenesis Receptor Kinases in Brassinosteroid Signaling | en_US |
dc.type | Research Article | en_US |
dc.description.peerreview | Yes | en_US |
dc.description.peerreviewnotes | http://www.plosgenetics.org/static/editorial#peer | en_US |
dc.identifier.doi | 10.1371/journal.pgen.1002452 | en_US |
dc.rights.requestable | false | en_US |