PaeExo IX: A unique deoxyribonuclease from Pseudomonas aeruginosa active in the presence of EDTA
Abstract
A new deoxyribonuclease, PaeExo IX, has been purified to electrophoretic homogeneity from extracts of Pseudomonas aeruginosa strain PAO. This enzyme, which is active in the presence of EDTA, is equally efficient in hydrolyzing native and heat-denatured DNA to acid-soluble products. The enzyme is partially or totally inhibited by the presence of several divalent cations. The active protein has a molecular weight of 1.6 +/= 0.1 x 10^5 and is composed of two nonidentical polypeptides with molecular weights of 78,000 and 69,000.
Citation
Scurlock, T. R., & Miller, R. V. (1979). PaeExo IX: A unique deoxyribonuclease from Pseudomonas aeruginosa active in the presence of EDTA. Nucleic Acids Research, 7(1), 167-177. https://doi.org/10.1093/nar/7.1.167