dc.contributor.author | Elasri, Mohamed O. | |
dc.contributor.author | Miller, Robert V. | |
dc.date.accessioned | 2015-10-16T20:48:31Z | |
dc.date.available | 2015-10-16T20:48:31Z | |
dc.date.issued | 1999-05 | |
dc.identifier | okds_Miller_AEM_1999-05.pdf | |
dc.identifier.citation | Elasri, M. O., & Miller, R. V. (1999). Study of the response of a biofilm bacterial community to UV radiation. Applied and Environmental Microbiology, 65(5), 2025-2031. https://doi.org/10.1128/AEM.65.5.2025-2031.1999 | |
dc.identifier.uri | https://hdl.handle.net/11244/19930 | |
dc.description.abstract | We have developed a bioluminescent whole-cell biosensor that can be incorporated into biofilm ecosystems. RM4440 is a Pseudomonas aeruginosa FRD1 derivative that carries a plasmid-based recA-luxCDABE fusion. We immobilized RM4440 in an alginate matrix to simulate a biofilm, and we studied its response to UV radiation damage. The biofilm showed a protective property by physical shielding against UV C, UV B, and UV A. Absorption of UV light by the alginate matrix translated into a higher survival rate than observed with planktonic cells at similar input fluences. UV A was shown to be effectively blocked by the biofilm matrix and to have no detectable effects on cells contained in the biofilm. However, in the presence of photosensitizers (i.e., psoralen), UV A was effective in inducing light production and cell death. RM4440 has proved to be a useful tool to study microbial communities in a noninvasive manner. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | American Society for Microbiology | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Study of the response of a biofilm bacterial community to UV radiation | |
osu.filename | okds_Miller_AEM_1999-05.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1128/AEM.65.5.2025-2031.1999 | |
dc.description.department | Microbiology and Molecular Genetics | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | alginates | |
dc.subject.keywords | biofilms: growth & development | |
dc.subject.keywords | biofilms: radiation effects | |
dc.subject.keywords | ecosystem | |
dc.subject.keywords | glucuronic acid | |
dc.subject.keywords | hexuronic acids | |
dc.subject.keywords | ultraviolet rays | |
dc.subject.keywords | pseudomonas aeruginosa: genetics | |
dc.subject.keywords | pseudomonas aeruginosa: physiology | |
dc.subject.keywords | pseudomonas aeruginosa: radiation effects | |