| dc.contributor.author | Ogunseitan, O. A. | |
| dc.contributor.author | Sayler, G. S. | |
| dc.contributor.author | Miller, Robert V. | |
| dc.date.accessioned | 2015-10-16T20:48:29Z | |
| dc.date.available | 2015-10-16T20:48:29Z | |
| dc.date.issued | 1992-06 | |
| dc.identifier | okds_Miller_AEM_1992-06.pdf | |
| dc.identifier.citation | Ogunseitan, O. A., Sayler, G. S., & Miller, R. V. (1992). Application of DNA probes to analysis of bacteriophage distribution patterns in the environment. Applied and Environmental Microbiology, 58(6), 2046-2052. https://doi.org/10.1128/aem.58.6.2046-2052.1992 | |
| dc.identifier.uri | https://hdl.handle.net/11244/19926 | |
| dc.description.abstract | Radiolabeled bacteriophage DNA probes have been used in this study to determine the distribution of Pseudomonas aeruginosa-infecting bacteriophages in natural samples of lake water, sediment, soil, and sewage. The sensitivity of detection of bacteriophage with the DNA probes was between 103 and 104 PFU and 106 to 107 CFU of lysogenized bacteria detectable with a homologous phage DNA probe. Analyses of environmental samples suggest that up to 40% of P. aeruginosa in natural ecosystems contain DNA sequences homologous to phage genomes. By using different bacteriophage DNA probes, the diversity of the bacteriophage population in sewage was estimated to be higher than that in other natural samples. The indication that transducing phages and prophages are widely distributed in the Pseudomonas populations investigated has considerable implications for the frequency of natural gene transfer by transduction and of lysogenic conversion of host bacteria in natural ecosystems. | |
| dc.format | application/pdf | |
| dc.language | en_US | |
| dc.publisher | American Society for Microbiology | |
| dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
| dc.title | Application of DNA probes to analysis of bacteriophage distribution patterns in the environment | |
| osu.filename | okds_Miller_AEM_1992-06.pdf | |
| dc.description.peerreview | Peer reviewed | |
| dc.identifier.doi | 10.1128/aem.58.6.2046-2052.1992 | |
| dc.description.department | Microbiology and Molecular Genetics | |
| dc.type.genre | Article | |
| dc.type.material | Text | |
