dc.contributor.author | Palmer, Guy H. | |
dc.contributor.author | Kocan, Katherine M. | |
dc.contributor.author | Barron, Selwyn J. | |
dc.contributor.author | Hair, Jakie A. | |
dc.contributor.author | Barbet, Anthony F. | |
dc.contributor.author | Davis, William C. | |
dc.contributor.author | McGuire, Travis C. | |
dc.date.accessioned | 2015-10-16T20:48:25Z | |
dc.date.available | 2015-10-16T20:48:25Z | |
dc.date.issued | 1985-12 | |
dc.identifier | okds_Kocan_IAI_1985-12.pdf | |
dc.identifier.citation | Palmer, G. H., Kocan, K. M., Barron, S. J., Hair, J. A., Barbet, A. F., Davis, W. C., & McGuire, T. C. (1985). Presence of common antigens, including major surface protein epitopes, between the cattle (intraerythrocytic) and tick stages of Anaplasma marginale. Infection and Immunity, 50(3), 881-886. https://doi.org/10.1128/iai.50.3.881-886.1985 | |
dc.identifier.uri | https://hdl.handle.net/11244/19918 | |
dc.description.abstract | Epitopes of major surface proteins of the intraerythrocytic cattle stage of Anaplasma marginale were demonstrated in the midgut stage of the organism within the infective tick host Dermacentor andersoni. These proteins were common to all A. marginale isolates tested and at all stages of parasitemia. Sera from cattle immunized with the tick midgut stage of A. marginale immunoprecipitated multiple-erythrocyte-stage proteins, as demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The major proteins recognized (primarily >14 and <200 kilodaltons [kDa]) included two major-erythrocyte-stage surface proteins of 36 and 105 kDa molecular size. To confirm the presence of common tick and erythrocyte A. marginale antigens with the immunized cattle sera, we purified the 36-kDa erythrocyte-stage protein by monoclonal immunoaffinity chromatography and developed an enzyme-linked immunosorbent assay based on the purified protein. All sera from cattle immunized with tick-stage A. marginale and cattle infected with various isolates of A. marginale developed antibodies to the 36-kDa protein. The potential immunoprophylactic, diagnostic, and epidemiologic value of the major epitopes common to both the invertebrate and mammalian stages of A. marginale, especially the 36-kDa protein, is discussed. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | American Society for Microbiology | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Presence of common antigens, including major surface protein epitopes, between the cattle (intraerythrocytic) and tick stages of Anaplasma marginale | |
osu.filename | okds_Kocan_IAI_1985-12.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1128/iai.50.3.881-886.1985 | |
dc.description.department | Veterinary Pathology | |
dc.description.department | Medicine and Surgery | |
dc.description.department | Entomology | |
dc.type.genre | Article | |
dc.type.material | Text | |