dc.contributor.author | Preiter, Karen | |
dc.contributor.author | Brooks, David M. | |
dc.contributor.author | Penaloza-Vazquez, Alejandro | |
dc.contributor.author | Sreedharan, Aswathy | |
dc.contributor.author | Bender, Carol L. | |
dc.contributor.author | Kunkel, Barbara N. | |
dc.date.accessioned | 2015-10-16T20:47:43Z | |
dc.date.available | 2015-10-16T20:47:43Z | |
dc.date.issued | 2005-11 | |
dc.identifier | okds_Bender_JB_2005-11.pdf | |
dc.identifier.citation | Preiter, K., Brooks, D. M., Penaloza-Vazquez, A., Sreedharan, A., Bender, C. L., & Kunkel, B. N. (2005). Novel virulence gene of Pseudomonas syringae pv. tomato strain DC3000. Journal of Bacteriology, 187(22), 7805-7814. https://doi.org/10.1128/JB.187.22.7805-7814.2005 | |
dc.identifier.uri | https://hdl.handle.net/11244/19774 | |
dc.description.abstract | Previously, we conducted a mutant screen of Pseudomonas syringae pv. tomato strain DC3000 to identify genes that contribute to virulence on Arabidopsis thaliana plants. Here we describe the characterization of one mutant strain, DB4H2, which contains a single Tn5 insertion in PSPTO3576, an open reading frame that is predicted to encode a protein belonging to the TetR family of transcriptional regulators. We demonstrate that PSPTO3576 is necessary for virulence in DC3000 and designate the encoded protein TvrR (TetR-like virulence regulator). TvrR, like many other TetR-like transcriptional regulators, negatively regulates its own expression. Despite the presence of a putative HrpL binding site in the tvrR promoter region, tvrR is not regulated by HrpL, an alternative sigma factor that regulates the expression of many known DC3000 virulence genes. tvrR mutant strains grow comparably to wild-type DC3000 in culture and possess an intact type III secretion system. However, tvrR mutants do not cause disease symptoms on inoculated A. thaliana and tomato plants, and their growth within plant tissue is significantly impaired. We demonstrate that tvrR mutant strains are able to synthesize coronatine (COR), a phytotoxin required for virulence of DC3000 on A. thaliana. Given that tvrR mutant strains are not defective for type III secretion or COR production, tvrR appears to be a novel virulence factor required for a previously unexplored process that is necessary for pathogenesis. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | American Society for Microbiology | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Novel virulence gene of Pseudomonas syringae pv. tomato strain DC3000 | |
osu.filename | okds_Bender_JB_2005-11.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1128/JB.187.22.7805-7814.2005 | |
dc.description.department | Entomology and Plant Pathology | |
dc.type.genre | Article | |
dc.type.material | Text | |