| dc.contributor.author | Preston, Lori A. | |
| dc.contributor.author | Wong, T. Y. | |
| dc.contributor.author | Bender, Carol L. | |
| dc.contributor.author | Schiller, Neal L. | |
| dc.date.accessioned | 2015-10-16T20:47:43Z | |
| dc.date.available | 2015-10-16T20:47:43Z | |
| dc.date.issued | 2000-11 | |
| dc.identifier | okds_Bender_JB_2000-11.pdf | |
| dc.identifier.citation | Preston, L. A., Wong, T. Y., Bender, C. L., & Schiller, N. L. (2000). Characterization of alginate lyase from Pseudomonas syringae pv. syringae. Journal of Bacteriology, 182(21), 6268-6271. https://doi.org/10.1128/JB.182.21.6268-6271.2000 | |
| dc.identifier.uri | https://hdl.handle.net/11244/19772 | |
| dc.description.abstract | The gene encoding alginate lyase (algL) in Pseudomonas syringae pv. syringae was cloned, sequenced, and overexpressed in Escherichia coli. Alginate lyase activity was optimal when the pH was 7.0 and when assays were conducted at 42°C in the presence of 0.2 M NaCl. In substrate specificity studies, AlgL from P. syringae showed a preference for deacetylated polymannuronic acid. Sequence alignment with other alginate lyases revealed conserved regions within AlgL likely to be important for the structure and/or function of the enzyme. Site-directed mutagenesis of histidine and tryptophan residues at positions 204 and 207, respectively, indicated that these amino acids are critical for lyase activity. | |
| dc.format | application/pdf | |
| dc.language | en_US | |
| dc.publisher | American Society for Microbiology | |
| dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
| dc.title | Characterization of alginate lyase from Pseudomonas syringae pv. Syringae | |
| osu.filename | okds_Bender_JB_2000-11.pdf | |
| dc.description.peerreview | Peer reviewed | |
| dc.identifier.doi | 10.1128/JB.182.21.6268-6271.2000 | |
| dc.description.department | Entomology and Plant Pathology | |
| dc.type.genre | Article | |
| dc.type.material | Text | |
