dc.contributor.author | Keith, Lisa M. W. | |
dc.contributor.author | Bender, Carol L. | |
dc.date.accessioned | 2015-10-16T20:47:43Z | |
dc.date.available | 2015-10-16T20:47:43Z | |
dc.date.issued | 1999-12 | |
dc.identifier | okds_Bender_JB_1999-12.pdf | |
dc.identifier.citation | Keith, L. M. W., & Bender, C. L. (1999). AlgT (sigma^22) controls alginate production and tolerance to environmental stress in Pseudomonas syringae. Journal of Bacteriology, 181(23), 7176-7184. https://doi.org/10.1128/JB.181.23.7176-7184.1999 | |
dc.identifier.uri | https://hdl.handle.net/11244/19771 | |
dc.description.abstract | Pseudomonas aeruginosa and the phytopathogen P. syringae produce the exopolysaccharide alginate, which is a copolymer of D-mannuronic and L-guluronic acids. One of the key regulatory genes controlling alginate biosynthesis in P. aeruginosa is algT, which encodes the alternate sigma factor, s22. In the present study, the algT gene product from P. syringae pv. syringae showed 90% amino acid identity with its P. aeruginosa counterpart, and sequence analysis of the region flanking algT in P. syringae revealed the presence of nadB, mucA, and mucB in an arrangement virtually identical to that of P. aeruginosa. An algT mutant of P. syringae was defective in alginate production but could be complemented with wild-type algT from P. syringae or P. aeruginosa when expressed in trans. The algT mutant also displayed increased sensitivity to heat, paraquat, and hydrogen peroxide (H2O2); the latter two compounds are known to generate reactive oxygen intermediates. Signals for activation of algT gene expression in P. syringae were investigated with an algT | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | American Society for Microbiology | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | AlgT (sigma^22) controls alginate production and tolerance to environmental stress in Pseudomonas syringae | |
osu.filename | okds_Bender_JB_1999-12.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1128/JB.181.23.7176-7184.1999 | |
dc.description.department | Entomology and Plant Pathology | |
dc.type.genre | Article | |
dc.type.material | Text | |