Study of the Bovine Pulmonary Response to Pasteurella Haemolytica. I. Pulmonary Macrophage Response. II. Specificity of Immunoglobulins Isolated from the Bovine Lung

Abstract

This thesis contains two chapters. These chapters were written to be complete and stand alone so tl1at they may be submitted independently for publication in the American Journal of Veterinary Research. Preceding the first chapter is an introduction which was written to introduce the overall theme of the thesis. A summary of the overall thesis follows the references of the second chapter. The research described here represents many hours spent trying to develop the techniques necessary to accomplish the objectives of the research proposal. There were basically three techniques that needed to be developed. These were: a method of harvesting pulmonary macrophages from the bovine lung and maintaining them in tissue culture; a method of incorporating 14c labeled amino acids into P. haemolytica; and a method for the isolation and purification of immunoglobulins from the bovine lung. The method described for the harvest of pulmonary macrophages is complete and should be followed easily. The method described for the incorporation of 14c labeled amino acids might be further investigated to increase the percent incorporation of the isotope.