Serological Detection of Peanut Stripe Virus

Abstract

The focus of this study was to develop monoclonal and polyclonal antibodies to peanut stripe virus (PStV) . These antibodies were then characterized and compared for the ability to detect PStV by various serological methods in both peanut leaf and seed tissues. The serological methods used were enzyme-linked immunosorbent assay, dotimmunobinding assay, and Western blotting. Methods for the detection of PStV were then applied to the problem of finding resistance to PStV in peanut germ plasm lines and the development of a peanut seed assay for the detection of PStV. For both applications the serological detection methods developed proved invaluable in the discovery of resistance and the development of a seed detection assay. The findings of this study should provide a basis for the standardization of detection assays and the control of PStV.