dc.date.accessioned | 2015-07-23T15:36:06Z | |
dc.date.available | 2015-07-23T15:36:06Z | |
dc.date.issued | 2014-08-05 | |
dc.identifier | pat8795988 | |
dc.identifier.uri | https://hdl.handle.net/11244/15435 | |
dc.description.abstract | Functional shRNA is produced from an expression vector prepared by selecting a two primer design in which the primers are less than about 50 nucleotides in length, annealing and extending the primers using primer extension, digesting the primer extension product and inserting the digestion product into a suitable vector. When the shRNA vectors are inserted into a cell, shRNA transcribed from the vectors modulates gene activity within the cell. | |
dc.format | application/pdf | |
dc.format.extent | 23 pages | |
dc.language | en_US | |
dc.publisher | U.S. Patent and Trademark Office | |
dc.title | Primer-extension Based Method for the Generation of Sirna/mirna Expression Vectors | |
dc.type | text | |
osu.filename | pat8795988.pdf | |
osu.accesstype | Open Access | |
dc.type.genre | Patent | |
dc.contributor.inventor | Liu, Lin | |
dc.contributor.inventor | Gou, Deming | |
dc.identifier.patentID | 8,795,988 | |
dc.date.filed | 2008-08-15 | |
dc.contributor.assignee | Board of Regents/Oklahoma State University and the A & M Colleges | |
dc.subject.primaryusclass | 435/91.1 | |
dc.subject.cpcclasses | C12N 15/10 (20130101) | |
dc.subject.cpcclasses | C12N 15/111 (20130101) | |
dc.subject.cpcclasses | C12N 15/64 (20130101) | |
dc.subject.cpcclasses | C12N 15/66 (20130101) | |
dc.subject.cpcclasses | C12N 2310/111 (20130101) | |
dc.subject.cpcclasses | C12N 2310/14 (20130101) | |
dc.subject.cpcclasses | C12N 2310/53 (20130101) | |
dc.subject.cpcclasses | C12N 2330/30 (20130101) | |