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dc.date.accessioned2015-07-23T15:36:06Z
dc.date.available2015-07-23T15:36:06Z
dc.date.issued2014-08-05
dc.identifierpat8795988
dc.identifier.urihttps://hdl.handle.net/11244/15435
dc.description.abstractFunctional shRNA is produced from an expression vector prepared by selecting a two primer design in which the primers are less than about 50 nucleotides in length, annealing and extending the primers using primer extension, digesting the primer extension product and inserting the digestion product into a suitable vector. When the shRNA vectors are inserted into a cell, shRNA transcribed from the vectors modulates gene activity within the cell.
dc.formatapplication/pdf
dc.format.extent23 pages
dc.languageen_US
dc.publisherU.S. Patent and Trademark Office
dc.titlePrimer-extension Based Method for the Generation of Sirna/mirna Expression Vectors
dc.typetext
osu.filenamepat8795988.pdf
osu.accesstypeOpen Access
dc.type.genrePatent
dc.contributor.inventorLiu, Lin
dc.contributor.inventorGou, Deming
dc.identifier.patentID8,795,988
dc.date.filed2008-08-15
dc.contributor.assigneeBoard of Regents/Oklahoma State University and the A & M Colleges
dc.subject.primaryusclass435/91.1
dc.subject.cpcclassesC12N 15/10 (20130101)
dc.subject.cpcclassesC12N 15/111 (20130101)
dc.subject.cpcclassesC12N 15/64 (20130101)
dc.subject.cpcclassesC12N 15/66 (20130101)
dc.subject.cpcclassesC12N 2310/111 (20130101)
dc.subject.cpcclassesC12N 2310/14 (20130101)
dc.subject.cpcclassesC12N 2310/53 (20130101)
dc.subject.cpcclassesC12N 2330/30 (20130101)


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