Sterilization of hydrogen peroxide resistant bacterial spores with stabilized chlorine dioxide
Date
2015-04-17Author
Friedline, Anthony
Zachariah, Malcolm
Middaugh, Amy
Heiser, Matt
Khanna, Neeraj
Vaishampayan, Parag
Rice, Charles V.
Metadata
Show full item recordAbstract
Bacillus pumilus SAFR-032 spores isolated from a clean room environment are known to exhibit enhanced resistance to peroxide, desiccation, UV radiation and chemical disinfection than other spore-forming bacteria. The survival of B. pumilus SAFR-032 spores to standard clean room sterilization practices requires development of more stringent disinfection agents. Here, we report the effects of a stabilized chlorine dioxide-based biocidal agent against spores of B. pumilus SAFR-032 and Bacillus subtilis ATCC 6051. Viability was determined via CFU measurement after exposure. Chlorine dioxide demonstrated efficacy towards sterilization of spores of B. pumilus SAFR-032 equivalent or better than exposure to hydrogen peroxide. These results indicate efficacy of chlorine dioxide delivered through a stabilized chlorine dioxide product as a means of sterilization of peroxide- and UV-resistant spores.
Citation
Friedline, Anthony, et al. Sterilization of hydrogen peroxide resistant bacterial spores with stabilized chlorine dioxide. AMB Express 2015, 5:24
Sponsorship
This work is supported by the National Institutes of Health (1R01GM090064-01), a NASA EPSCoR Research Infrastructure Development (RID) grant NN07AL49A, and the University of Oklahoma.