Elevated Levels of the Vesicular Monoamine Transporter and a Novel Repetitive Behavior in the Drosophila Model of Fragile X Syndrome

John M. Tauber; Phillip A. Vanlandingham; Bing Zhang

dc.contributor.author	John M. Tauber	en_US
dc.contributor.author	Phillip A. Vanlandingham	en_US
dc.contributor.author	Bing Zhang	en_US
dc.date.accessioned	2015-01-23T17:17:33Z
dc.date.accessioned	2016-03-30T15:35:45Z
dc.date.available	2015-01-23T17:17:33Z
dc.date.available	2016-03-30T15:35:45Z
dc.date.issued	2011-11-02	en_US
dc.identifier.citation	Tauber JM, Vanlandingham PA, Zhang B (2011) Elevated Levels of the Vesicular Monoamine Transporter and a Novel Repetitive Behavior in the Drosophila Model of Fragile X Syndrome. PLoS ONE 6(11): e27100. doi:10.1371/journal.pone.0027100	en_US
dc.identifier.uri	https://hdl.handle.net/11244/14070
dc.description	We thank Drs. Yong Q. Zhang and Kendal Broadie for providing the w; dfmr183M/TM6B, Tb stock, and Dr. Thomas Jongens for providing the w; dfmr13/TM6C, Sb, wild-type rescue (Control) and frameshift rescue (FS) stocks. We also thank Dr. David Krantz for his advice on the use of acetic acid in dissolving reserpine and for providing the antibody to dVMAT, Drs. Michael Tranfaglia, Randy Hewes, Rosemary Knapp, and David McCauley for constructive comments on the manuscript, Dr. Lauren Ritterhouse for assistance in the statistical analysis of the data, Logan Cox for assistance on fly husbandry, and Emily Kumimoto, Rod Kumimoto, and Sunetra Das for their instruction and assistance with RNA isolation and real-time PCR. The MPEP drug was a generous gift of Dr. Michael Tranfaglia from the FRAXA Research Foundation. J.M.T. received the inaugural Vad Foundation Award for Outstanding Undergraduate Thesis for this research. We wish to thank Dr. Vijay Vad and his Foundation for his support.	en_US
dc.description	Conceived and designed the experiments: JMT BZ. Performed the experiments: JMT PAV. Analyzed the data: JMT PAV BZ. Contributed reagents/materials/analysis tools: JMT PAV BZ. Wrote the paper: JMT BZ. Edited the text: PAV.	en_US
dc.description.abstract	Fragile X Syndrome (FXS) is characterized by mental impairment and autism in humans, and it often features hyperactivity and repetitive behaviors. The mechanisms for the disease, however, remain poorly understood. Here we report that the dfmr1 mutant in the Drosophila model of FXS grooms excessively, which may be regulated differentially by two signaling pathways. Blocking metabotropic glutamate receptor signaling enhances grooming in dfmr1 mutant flies, whereas blocking the vesicular monoamine transporter (VMAT) suppresses excessive grooming. dfmr1 mutant flies also exhibit elevated levels of VMAT mRNA and protein. These results suggest that enhanced monoamine signaling correlates with repetitive behaviors and hyperactivity associated with FXS.	en_US
dc.language.iso	en_US	en_US
dc.publisher	PLos One	en_US
dc.relation.ispartofseries	PLoS ONE 6(11):e27100	en_US
dc.relation.uri	http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0027100	en_US
dc.rights	Attribution 3.0 United States	en_US
dc.rights.uri	https://creativecommons.org/licenses/by/3.0/us/	en_US
dc.subject	PLOS	en_US
dc.subject	Public Library of Science	en_US
dc.subject	Open Access	en_US
dc.subject	Open-Access	en_US
dc.subject	Science	en_US
dc.subject	Medicine	en_US
dc.subject	Biology	en_US
dc.subject	Research	en_US
dc.subject	Peer-review	en_US
dc.subject	Inclusive	en_US
dc.subject	Interdisciplinary	en_US
dc.subject	Ante-disciplinary	en_US
dc.subject	Physics	en_US
dc.subject	Chemistry	en_US
dc.subject	Engineering	en_US
dc.title	Elevated Levels of the Vesicular Monoamine Transporter and a Novel Repetitive Behavior in the Drosophila Model of Fragile X Syndrome	en_US
dc.type	Research Article	en_US
dc.description.peerreview	Yes	en_US
dc.description.peerreviewnotes	http://www.plosone.org/static/editorial#peer	en_US
dc.identifier.doi	10.1371/journal.pone.0027100	en_US
dc.rights.requestable	false	en_US