dc.contributor.author | Shuanghe Cao | en_US |
dc.contributor.author | Roderick W. Kumimoto | en_US |
dc.contributor.author | Chamindika L. Siriwardana | en_US |
dc.contributor.author | Jan R. Risinger | en_US |
dc.contributor.author | Ben F. Holt III | en_US |
dc.date.accessioned | 2015-01-23T17:17:33Z | |
dc.date.accessioned | 2016-03-30T15:35:46Z | |
dc.date.available | 2015-01-23T17:17:33Z | |
dc.date.available | 2016-03-30T15:35:46Z | |
dc.date.issued | 2011-06-30 | en_US |
dc.identifier.citation | Cao S, Kumimoto RW, Siriwardana CL, Risinger JR, Holt BF III (2011) Identification and Characterization of NF-Y Transcription Factor Families in the Monocot Model Plant Brachypodium distachyon. PLoS ONE 6(6): e21805. doi:10.1371/journal.pone.0021805 | en_US |
dc.identifier.uri | https://hdl.handle.net/11244/14069 | |
dc.description | We thank our undergraduate students Krystal Gayler, Krishna Suthar, and Kristen Zahnow for assistance in the lab. | en_US |
dc.description | Conceived and designed the experiments: BFH RWK SC CLS. Performed the experiments: BFH SC RWK CLS JRR. Analyzed the data: BFH SC RWK. Contributed reagents/materials/analysis tools: BFH SC RWK CLS JRR. Wrote the paper: BFH. | en_US |
dc.description.abstract | Background Nuclear Factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. Using the dicot plant model system Arabidopsis thaliana (Arabidopsis), NF-Y were previously shown to control a variety of agronomically important traits, including drought tolerance, flowering time, and seed development. The aim of the current research was to identify and characterize NF-Y families in the emerging monocot model plant Brachypodium distachyon (Brachypodium) with the long term goal of assisting in the translation of known dicot NF-Y functions to the grasses. Methodology/Principal Findings We identified, annotated, and further characterized 7 NF-YA, 17 NF-YB, and 12 NF-YC proteins in Brachypodium (BdNF-Y). By examining phylogenetic relationships, orthology predictions, and tissue-specific expression patterns for all 36 BdNF-Y, we proposed numerous examples of likely functional conservation between dicots and monocots. To test one of these orthology predictions, we demonstrated that a BdNF-YB with predicted orthology to Arabidopsis floral-promoting NF-Y proteins can rescue a late flowering Arabidopsis mutant. Conclusions/Significance The Brachypodium genome encodes a similar complement of NF-Y to other sequenced angiosperms. Information regarding NF-Y phylogenetic relationships, predicted orthologies, and expression patterns can facilitate their study in the grasses. The current data serves as an entry point for translating many NF-Y functions from dicots to the genetically tractable monocot model system Brachypodium. In turn, studies of NF-Y function in Brachypodium promise to be more readily translatable to the agriculturally important grasses. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | PLos One | en_US |
dc.relation.ispartofseries | PLoS ONE 6(6):e21805 | en_US |
dc.relation.uri | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0021805 | en_US |
dc.rights | Attribution 3.0 United States | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/3.0/us/ | en_US |
dc.subject | PLOS | en_US |
dc.subject | Public Library of Science | en_US |
dc.subject | Open Access | en_US |
dc.subject | Open-Access | en_US |
dc.subject | Science | en_US |
dc.subject | Medicine | en_US |
dc.subject | Biology | en_US |
dc.subject | Research | en_US |
dc.subject | Peer-review | en_US |
dc.subject | Inclusive | en_US |
dc.subject | Interdisciplinary | en_US |
dc.subject | Ante-disciplinary | en_US |
dc.subject | Physics | en_US |
dc.subject | Chemistry | en_US |
dc.subject | Engineering | en_US |
dc.title | Identification and Characterization of NF-Y Transcription Factor Families in the Monocot Model Plant Brachypodium distachyon | en_US |
dc.type | Research Article | en_US |
dc.description.peerreview | Yes | en_US |
dc.description.peerreviewnotes | http://www.plosone.org/static/editorial#peer | en_US |
dc.identifier.doi | 10.1371/journal.pone.0021805 | en_US |
dc.rights.requestable | false | en_US |