dc.contributor.author | J. Jacob Parnell | en_US |
dc.contributor.author | Giovanni Rompato | en_US |
dc.contributor.author | Leigh C. Latta IV | en_US |
dc.contributor.author | Michael E. Pfrender | en_US |
dc.contributor.author | Joy D. Van Nostrand | en_US |
dc.contributor.author | Zhili He | en_US |
dc.contributor.author | Jizhong Zhou | en_US |
dc.contributor.author | Gary Andersen | en_US |
dc.contributor.author | Patti Champine | en_US |
dc.contributor.author | Balasubramanian Ganesan | en_US |
dc.contributor.author | Bart C. Weimer | en_US |
dc.date.accessioned | 2015-01-23T17:17:31Z | |
dc.date.accessioned | 2016-03-30T15:31:06Z | |
dc.date.available | 2015-01-23T17:17:31Z | |
dc.date.available | 2016-03-30T15:31:06Z | |
dc.date.issued | 2010-09-23 | en_US |
dc.identifier.citation | Parnell JJ, Rompato G, Latta LC IV, Pfrender ME, Van Nostrand JD, et al. (2010) Functional Biogeography as Evidence of Gene Transfer in Hypersaline Microbial Communities. PLoS ONE 5(9): e12919. doi:10.1371/journal.pone.0012919 | en_US |
dc.identifier.uri | https://hdl.handle.net/11244/14065 | |
dc.description | We are grateful to David Naftz and the United States Geological Survey for access to sampling sites, Chris Garrard and Robert Baskin for geospatial images, and Ethan White for statistical input. | en_US |
dc.description | Conceived and designed the experiments: JJP GR MEP BCW. Performed the experiments: JJP GR JDVN. Analyzed the data: JJP LCL MEP JDVN BG. Contributed reagents/materials/analysis tools: JJP GR LCL MEP JDVN ZH JZ GLA BCW. Wrote the paper: JJP MEP PC BCW. | en_US |
dc.description.abstract | BackgroundHorizontal gene transfer (HGT) plays a major role in speciation and evolution of bacteria and archaea by controlling gene distribution within an environment. However, information that links HGT to a natural community using relevant population-genetics parameters and spatial considerations is scarce. The Great Salt Lake (Utah, USA) provides an excellent model for studying HGT in the context of biogeography because it is a contiguous system with dispersal limitations due to a strong selective salinity gradient. We hypothesize that in spite of the barrier to phylogenetic dispersal, functional characteristics—in the form of HGT—expand beyond phylogenetic limitations due to selective pressure.Methodology and ResultsTo assay the functional genes and microorganisms throughout the GSL, we used a 16S rRNA oligonucleotide microarray (Phylochip) and a functional gene array (GeoChip) to measure biogeographic patterns of nine microbial communities. We found a significant difference in biogeography based on microarray analyses when comparing Sørensen similarity values for presence/absence of function and phylogeny (Student's t-test; p = 0.005).Conclusion and SignificanceBiogeographic patterns exhibit behavior associated with horizontal gene transfer in that informational genes (16S rRNA) have a lower similarity than functional genes, and functional similarity is positively correlated with lake-wide selective pressure. Specifically, high concentrations of chromium throughout GSL correspond to an average similarity of chromium resistance genes that is 22% higher than taxonomic similarity. This suggests active HGT may be measured at the population level in microbial communities and these biogeographic patterns may serve as a model to study bacteria adaptation and speciation. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | PLos One | en_US |
dc.relation.ispartofseries | PLoS ONE 5(9):e12919 | en_US |
dc.relation.uri | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0012919 | en_US |
dc.rights | Attribution 3.0 United States | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/3.0/us/ | en_US |
dc.subject | PLOS | en_US |
dc.subject | Public Library of Science | en_US |
dc.subject | Open Access | en_US |
dc.subject | Open-Access | en_US |
dc.subject | Science | en_US |
dc.subject | Medicine | en_US |
dc.subject | Biology | en_US |
dc.subject | Research | en_US |
dc.subject | Peer-review | en_US |
dc.subject | Inclusive | en_US |
dc.subject | Interdisciplinary | en_US |
dc.subject | Ante-disciplinary | en_US |
dc.subject | Physics | en_US |
dc.subject | Chemistry | en_US |
dc.subject | Engineering | en_US |
dc.title | Functional Biogeography as Evidence of Gene Transfer in Hypersaline Microbial Communities | en_US |
dc.type | Research Article | en_US |
dc.description.peerreview | Yes | en_US |
dc.description.peerreviewnotes | http://www.plosone.org/static/editorial#peer | en_US |
dc.identifier.doi | 10.1371/journal.pone.0012919 | en_US |
dc.rights.requestable | false | en_US |