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Elucidating the catalytic mechanism of malic enzyme via site-directed mutagenesis studies.
(2001)
Based on homology search results and the three-dimensional structures of malic enzymes, 12 highly conserved ionizable residues were selected as potential general acid/base in the malic enzyme reaction. Site-directed ...
Development and analysis of an expressed sequence tag database from Fusarium sporotrichioides.
(2001)
The comparison of four other fungal cDNA libraries sequenced in our lab, F. sporotrichioides library, two Neurospora crassa libraries and one Aspergillus nidulans library, shows that the percentage of biological function ...
Mechanism of ferric enterobactin transport through Escherichia coli FepA: The evolution of a bacterial venus flytrap.
(2001)
The TonB-dependent, energy dependent ferric siderophore transporters of the gram-negative bacterial outer membrane possess a unique architecture: Their N-termini fold into a globular domain that lodges within, and physically ...
Expression profiles and the identification of the clock-controlled genes in two time-of-day-specific cDNA libraries of Neurospora crassa.
(2001)
In the group of genes that were detected from both cDNA libraries, the highest three of the top ten highly expressed genes were clock-controlled genes (ccgs). That these ccgs are so highly expressed in Neurospora implies ...
Crystalline and amorphous phases in polymer electrolytes and model systems.
(2001)
A symmetry-based vibrational analysis is used to interpret bands in crystalline polymer-salt compounds. Vibrational spectroscopic data of crystalline polymer:salt complexes reveal the presence of dynamic coupling of the ...
Cell surface exposure and immunogenicity of foreign epitopes in outer membrane proteins.
(2001)
To evaluate the accessibility of epitopes to the extracellular environment, bacteria expressing LamB:V3 or OmpA:TOP chimeric protein were fluorescently labeled with mouse anti-gp120 (V3), and analyzed by cytofluorimetry. ...
The structure and function of YPD1, a histidine-containing phosphotransfer protein from yeast.
(2001)
Finally, through molecular modeling and structural comparisons, a general model of how YPD1 and response regulator domains might interact was proposed. It seems likely that YPD1 binds to response regulator domains through ...