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Elucidating the catalytic mechanism of malic enzyme via site-directed mutagenesis studies.
(2001)
Based on homology search results and the three-dimensional structures of malic enzymes, 12 highly conserved ionizable residues were selected as potential general acid/base in the malic enzyme reaction. Site-directed ...
Development and analysis of an expressed sequence tag database from Fusarium sporotrichioides.
(2001)
The comparison of four other fungal cDNA libraries sequenced in our lab, F. sporotrichioides library, two Neurospora crassa libraries and one Aspergillus nidulans library, shows that the percentage of biological function ...
Verification of microbial ethanol formation in humans by examination of postmortem indoleamine metabolites.
(2004)
Toxicological examination of fatal aviation accident victims routinely includes analysis of ethanol levels. Two distinct questions immediately arise when considering such analyses. The first is, can we stop ethanol production ...
MECHANISTIC INVESTIGATION OF ADDITIONS TO ALKENES
(2009)
We have conducted correlation studies on ten alkene addition reactions in this project in order to explore the substituent effects on alkene reactivity in these reactions. In these studies, we have correlated the relative ...
Interactions of organic S-nitroso and C-nitroso compounds with metalloporphyrins.
(2002)
Chapter 4 of this dissertation deals with the first isolable nitrone adducts of metalloporphyrins. Thus, the metalloporphyrin nitrone complexes (OEP)M(CO)(DMPO) (M = Ru, Os; DMPO = 5,5-dimethyl-1-pyrroline N-oxide) were ...
Effect of lipid modification and AcrA-lipid interactions on the structure and oligomerization of AcrA
(2009)
A wide range of antibacterial agents including antibiotics can be expelled out of the cell by AcrAB-TolC complex. In this complex, the membrane fusion protein AcrA is located in the periplasm and essential for drug efflux. ...
Design of new molecular dynamics global minimum search protocols for mapping energy landscapes and conformations of folded polypeptides and mini-proteins.
(2005)
First, a new MD search strategy called DIvergent Path (DIP) search simulation is developed in which the simulations start with several independent polypeptides having the same initial coordinates and temperatures but ...
TonB dependent transport processes through Escherichia coli FepA.
(2007)
One of the previously proposed mechanisms of TonB is called the "shuttle hypothesis" which states that TonB shuttles from the inner membrane to the outer membrane. The data presented here demonstrates that TonB does not ...
DIRECT METAGENOMIC DETECTION AND ANALYSIS OF PLANT VIRUSES USING AN UNBIASED HIGH-THROUGHPUT SEQUENCING APPROACH
(2009)
It is well established that plants, along with other life forms, often are infected by viral parasites that require the host cellular machinery for replication. Since the overwhelming majority of these viruses have been ...
Mechanisms of Serine Acetyltransferase and O-Acetylserine Sulfhydrylase
(2009)
The de novo biosynthesis of L-cysteine in enteric bacteria and higher plants is a two-step pathway. The first step is catalyzed by serine acetyltransferase (SAT), which converts acetyl CoA and L-serine to O-acetyl-L-serine ...