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Mechanism of saccharopine dehydrogenase: The last enzyme in the lysine biosynthetic pathway in Saccharomyces cerevisiae.
(2007)
A proton shuttle chemical mechanism is proposed on the basis of the pH dependence of kinetic parameters, dissociation constants for competitive inhibitors, and isotope effects. In the direction of lysine formation, once ...
Chemical Modeling of Unstable Intermediates in Heme-mediated NOx Biology
(2017-05-12)
This dissertation describes the preparation, characterization, and reactivity of several critical unstable intermediates of relevance to the global N-cycle. Their spectroscopic signatures and chemical reactivities were ...
Single-molecule spectroscopic investigation of the influence that charge-charge interactions, solvation, and confinement have on guest molecule rotational mobility and photostability within silica sol-gel host matrices.
(2006)
To investigate physical immobilization further, guest behavior was monitored within the more highly branched silica nanoparticle matrix, when covalently bonded to silica nanoparticles, and within pores templated by gold ...
Studies of the complexation reactions of polyether ionophore antibiotics monensin A and lasalocid A with lead(II), zinc, calcium and sodium cations.
(2000)
Lead-ionophore complexes of the form PbLX and PbL2 (L = monensin A or lasalocid A; X- = anion) were isolated and characterized by elemental analysis (X = Cl--) and ESI-MS (X = Cl --, NO3, CH3COO--). Changes in the UV-Vis ...
Mechanism of ferric enterobactin transport through Escherichia coli FepA: The evolution of a bacterial venus flytrap.
(2001)
The TonB-dependent, energy dependent ferric siderophore transporters of the gram-negative bacterial outer membrane possess a unique architecture: Their N-termini fold into a globular domain that lodges within, and physically ...
Imitating Nature: The Effects of Asymmetry and Strain on Copper and Iron Complexes with Schiff Base Ligands
(2013)
Metalloenzymes are incredibly complex "machines" which catalyze reactions essential for life. Metal ions are found in more than half of all known proteins; particularly, copper and iron are abundant in active sites performing ...
Local structures and conductivity in polyethylenimine and polyphosphazene polymer electrolytes.
(2002)
Linear polyethylenimine (LPEI) is structurally analogous to poly(ethylene oxide), with an N-H group in place of the ether oxygen. LPEI systems with dissolved lithium and sodium triflate were prepared and characterized via ...
Part I. Kinetic studies of the complexation reactions of nickel(II) with hydroxamic acids in aqueous medium. Part II. Kinetic studies of the formation and dissociation reactions of ionomycin and 2,6-dimethylheptane-3,5-dione with nickel(II) and magnesium(II) in 80 percent methanol-water medium.
(1997)
The second part of the study involved the reactions of nickel(II) and magnesium(II), with the ionophore ionomycin and the model compound 2,6-dimethyl-3,5-heptanedione (DMHD). A detailed analysis of the kinetic data for ...
PROBING THE MECHANISM OF SACCHAROPINE DEHYDROGENASE FROM Saccharomyces cerevisiae USING SITE-DIRECTED MUTAGENESIS
(2010)
Saccharopine dehydrogenase (SDH) catalyses the NAD-dependent oxidative deamination of saccharopine to give L-lysine and alpha-ketoglutarate. There are a number of conserved hydrophilic, ionizable residues in the active ...
SYNTHESIS OF STRUCTURAL MODELS OF NON-HEME DIOXYGENASES. MIMICS OF THE 3-His-1-CARBOXYLATE TETRAD
(2009)
Metalloenzymes catalyze various reactions and in particular dioxygenases insert dioxygen into organic substrates. Since these enzymes usually utilize histidines as ligands for the metal cofactor, we focused our attention ...