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Mechanism of saccharopine dehydrogenase: The last enzyme in the lysine biosynthetic pathway in Saccharomyces cerevisiae.
(2007)
A proton shuttle chemical mechanism is proposed on the basis of the pH dependence of kinetic parameters, dissociation constants for competitive inhibitors, and isotope effects. In the direction of lysine formation, once ...
Studies on selected enzymes of the alpha-aminoadipate pathway for lysine biosynthesis in Saccharomyces cerevisiae.
(2005)
Homocitrate synthase (HCS) catalyzes one of the regulated steps of the alpha-aminoadipate pathway for Lys biosynthesis in fungi. His-tagged HCS from Saccharomyces cerevisiae was purified to about 98% using a Ni-NTA resin ...
The kinetic and chemical mechanism of serine acetyltransferase from Haemophilus influenzae.
(2004)
The pH dependence of kinetic parameters was determined in both reaction directions in order to obtain information on the acid-base chemical mechanism of serine acetyltransferase from Haemophilus influenzae ( HiSAT). The ...
Site-directed mutagenesis as a probe to study the mechanism of sheep liver 6-phosphogluconate dehydrogenase.
(2006)
All mutant enzymes in the 2'-phosphate site exhibit an increase in KNADP that ranges from 6-fold to 80-fold. A 7-fold increase in the primary kinetic 13C-isotope effect minus 1 in all mutant enzymes indicates that the ...