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Site-directed mutagenesis as a probe to study the mechanism of sheep liver 6-phosphogluconate dehydrogenase.
(2006)
All mutant enzymes in the 2'-phosphate site exhibit an increase in KNADP that ranges from 6-fold to 80-fold. A 7-fold increase in the primary kinetic 13C-isotope effect minus 1 in all mutant enzymes indicates that the ...