Search
Now showing items 11-20 of 26
Mechanism of saccharopine dehydrogenase: The last enzyme in the lysine biosynthetic pathway in Saccharomyces cerevisiae.
(2007)
A proton shuttle chemical mechanism is proposed on the basis of the pH dependence of kinetic parameters, dissociation constants for competitive inhibitors, and isotope effects. In the direction of lysine formation, once ...
Mechanism of ferric enterobactin transport through Escherichia coli FepA: The evolution of a bacterial venus flytrap.
(2001)
The TonB-dependent, energy dependent ferric siderophore transporters of the gram-negative bacterial outer membrane possess a unique architecture: Their N-termini fold into a globular domain that lodges within, and physically ...
Testing recently-developed molecular dynamics protocols for structure prediction of polypeptides and prion proteins.
(2007)
Two MD protocols were recently developed that allow a polypeptide to search the PE surface in search of the global PE minimum, which should correspond to the experimental structure. alpha-helical secondary structures have ...
Sequencing and comparative genomics of Leptospira interrogans serovar pomona and Leptospira kirschneri serovar grippotyphosa.
(2007)
Virulence genes that include methyl-accepting chemotaxis protein, flagellar basal body-associated protein, flagellar motor switch protein, Lig A protein, thermolysin, multiple antibiotic resistance protein, acriflavine ...
Studies on selected enzymes of the alpha-aminoadipate pathway for lysine biosynthesis in Saccharomyces cerevisiae.
(2005)
Homocitrate synthase (HCS) catalyzes one of the regulated steps of the alpha-aminoadipate pathway for Lys biosynthesis in fungi. His-tagged HCS from Saccharomyces cerevisiae was purified to about 98% using a Ni-NTA resin ...
A comparison of the standard DNA shotgun sequencing approach and the exponential DNA amplification method for sequencing BAC, fosmid, plasmid, and mitochondrial DNA.
(2003)
Therefore, during the initial phase of my studies, I became well versed in the standard shotgun sequencing techniques and completed the sequence of six regions of the human and mouse genome totaling over 1 Mbp. Then, because ...
Multiple species comparative analysis of human chromosome 22 between markers D22S1687 and D22S419 and gene expression profiling in zebrafish.
(2006)
Major large scale insertions or deletions that resulted in gene number differences between human and chimpanzee were discovered in the IGLL and LCR22s within this region, with four human insertions from 6 Kb to 75 Kb and ...
The kinetic and chemical mechanism of serine acetyltransferase from Haemophilus influenzae.
(2004)
The pH dependence of kinetic parameters was determined in both reaction directions in order to obtain information on the acid-base chemical mechanism of serine acetyltransferase from Haemophilus influenzae ( HiSAT). The ...
Dissecting the reaction mechanism of sheep liver 6-phosphogluconate dehydrogenase.
(2000)
In the second part of the research, three additional mutants, S 128A, H 186A and N187A were characterized in the same manner as the K183 mutant enzymes. The decrease in the activity compared to the wild type enzyme is about ...
Genetic and biochemical characterization of hydroxamate transporters in Listeria monocytogenes.
(2007)
Listeria monocytogenes is a Gram-positive bacterial pathogen that can cause severe opportunistic infection in humans and animals, and iron is a virulence determinant in this pathogen. By sequence homologies, the genome of ...