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Studies on selected enzymes of the alpha-aminoadipate pathway for lysine biosynthesis in Saccharomyces cerevisiae.
(2005)
Homocitrate synthase (HCS) catalyzes one of the regulated steps of the alpha-aminoadipate pathway for Lys biosynthesis in fungi. His-tagged HCS from Saccharomyces cerevisiae was purified to about 98% using a Ni-NTA resin ...
Mechanism of saccharopine dehydrogenase: The last enzyme in the lysine biosynthetic pathway in Saccharomyces cerevisiae.
(2007)
A proton shuttle chemical mechanism is proposed on the basis of the pH dependence of kinetic parameters, dissociation constants for competitive inhibitors, and isotope effects. In the direction of lysine formation, once ...
Mechanism of ferric enterobactin transport through Escherichia coli FepA: The evolution of a bacterial venus flytrap.
(2001)
The TonB-dependent, energy dependent ferric siderophore transporters of the gram-negative bacterial outer membrane possess a unique architecture: Their N-termini fold into a globular domain that lodges within, and physically ...
Testing recently-developed molecular dynamics protocols for structure prediction of polypeptides and prion proteins.
(2007)
Two MD protocols were recently developed that allow a polypeptide to search the PE surface in search of the global PE minimum, which should correspond to the experimental structure. alpha-helical secondary structures have ...
Sequencing and comparative genomics of Leptospira interrogans serovar pomona and Leptospira kirschneri serovar grippotyphosa.
(2007)
Virulence genes that include methyl-accepting chemotaxis protein, flagellar basal body-associated protein, flagellar motor switch protein, Lig A protein, thermolysin, multiple antibiotic resistance protein, acriflavine ...
Genetic and biochemical characterization of hydroxamate transporters in Listeria monocytogenes.
(2007)
Listeria monocytogenes is a Gram-positive bacterial pathogen that can cause severe opportunistic infection in humans and animals, and iron is a virulence determinant in this pathogen. By sequence homologies, the genome of ...
Site-directed mutagenesis as a probe to study the mechanism of sheep liver 6-phosphogluconate dehydrogenase.
(2006)
All mutant enzymes in the 2'-phosphate site exhibit an increase in KNADP that ranges from 6-fold to 80-fold. A 7-fold increase in the primary kinetic 13C-isotope effect minus 1 in all mutant enzymes indicates that the ...
A bioinorganic approach to the study of the interactions of nitrogen oxides with manganese porphyrins and manganese- and cobalt-reconstituted myoglobin.
(2007)
This dissertation deals with the interaction of the biologically important nitrogen oxides (nitric oxide and nitrite) with manganese and ruthenium centers in small inorganic porphyrin and nonporphyrin complexes as well as ...
A comparison of the standard DNA shotgun sequencing approach and the exponential DNA amplification method for sequencing BAC, fosmid, plasmid, and mitochondrial DNA.
(2003)
Therefore, during the initial phase of my studies, I became well versed in the standard shotgun sequencing techniques and completed the sequence of six regions of the human and mouse genome totaling over 1 Mbp. Then, because ...
Multiple species comparative analysis of human chromosome 22 between markers D22S1687 and D22S419 and gene expression profiling in zebrafish.
(2006)
Major large scale insertions or deletions that resulted in gene number differences between human and chimpanzee were discovered in the IGLL and LCR22s within this region, with four human insertions from 6 Kb to 75 Kb and ...