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Elucidating the catalytic mechanism of malic enzyme via site-directed mutagenesis studies.
(2001)
Based on homology search results and the three-dimensional structures of malic enzymes, 12 highly conserved ionizable residues were selected as potential general acid/base in the malic enzyme reaction. Site-directed ...
Development and analysis of an expressed sequence tag database from Fusarium sporotrichioides.
(2001)
The comparison of four other fungal cDNA libraries sequenced in our lab, F. sporotrichioides library, two Neurospora crassa libraries and one Aspergillus nidulans library, shows that the percentage of biological function ...
Verification of microbial ethanol formation in humans by examination of postmortem indoleamine metabolites.
(2004)
Toxicological examination of fatal aviation accident victims routinely includes analysis of ethanol levels. Two distinct questions immediately arise when considering such analyses. The first is, can we stop ethanol production ...
TonB dependent transport processes through Escherichia coli FepA.
(2007)
One of the previously proposed mechanisms of TonB is called the "shuttle hypothesis" which states that TonB shuttles from the inner membrane to the outer membrane. The data presented here demonstrates that TonB does not ...
The role of membrane fusion proteins in transport of diverse substrates in bacteria.
(2007)
Multidrug resistance (MDR) efflux pumps are increasingly recognized as major contributors to resistance in clinically resistant isolates. MDR is associated with increased morbidity and mortality in hospitals and is a serious ...
Characterization of the second-half reaction of O-acetylserine sulfhydrylase-A and the reaction of O-acetylserine sulfhydrylase-B from Salmonella typhimurium.
(2004)
The O-acetylserine sulfhydrylase-B (OASS-B) was recently overexpressed and purified to electrophoretic homogeneity (>98% pure). Both the quantity and purity of the enzyme permit more kinetic and structurally based studies ...
Functional characterization of the phosphorelay protein MPR1 from Schizosaccharomyces pombe.
(2007)
The yeast two-hybrid assay indicates that MPR1 interacts with the response regulator domains of MAK3 (one of the three histidine kinases) and MCS4, but not with MAK1, MAK2 and PRR1. PRR1-RR weakly interacts with MAK1-HK, ...
Comparative analysis of human chromosome 22 CES-DGCR syntenic regions in chimpanzee, baboon, bovine, mouse and zebrafish and expression profiling in zebrafish early developmental stages using whole mount in situ hybridization.
(2006)
The final series of experiments were based on the earlier observation that 16 genes in the human chromosome 22 CES-DGCR region had reported expression but no detailed expression profiles while 6 others had no known expression ...
The working draft sequence of the Gram-positive bacteria Bacillus stearothermophilus strain 10 genome, its analysis, and annotation.
(2002)
A whole-genome random shotgun (WGS) sequencing method was used to obtain the working draft sequence. To date, over 99 percent of this approximately 3.4 Mbp Bacillus stearothermophilus genome has been sequenced from ...
A study of the interactions of nitric oxide and nitric oxide containing molecules with heme proteins.
(2006)
The interactions of nitric oxide (NO), nitrite and organic nitroso compounds with heme proteins are biologically relevant. The formation of adducts between these NO-containing species and myoglobin (Mb) has served as a ...