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    • Primer-extension Based Method for the Generation of Sirna/mirna Expression Vectors 

      Liu, Lin; Gou, Deming; Board of Regents/Oklahoma State University and the A & M Colleges (U.S. Patent and Trademark Office, 2014-08-05)
      Functional shRNA is produced from an expression vector prepared by selecting a two primer design in which the primers are less than about 50 nucleotides in length, annealing and extending the primers using primer extension, ...