Browsing by Author "Karr, Elizabeth"
Now showing items 1-18 of 18
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Bioactive Molecules Detection and Identification in Complex Biological Systems through Activity-Based Metabolomics and Proteomics
Ma, Hongyan (2020-07)The detection and identification of bioactive molecules (e.g. therapeutic small-molecule lead compounds, functional proteins) from complex mixtures is often a critical step in various biochemical and biomedical research ... -
THE BIOCHEMICAL CHARACTERIZATION OF TWO PHOSPHORELAY SIGNALING PROTEINS: YPD1 HOMOLOGS FROM SACCHAROMYCES CEREVISIAE AND CRYPTOCOCCUS NEOFORMANS
Kennedy, Emily (2016-08)Histidine-to-aspartate multi-step phosphorelay systems are used extensively by eukaryotes and bacteria to sense and respond to changes in their external environment and to control the regulation of crucial biological ... -
Biotic and abiotic chemical weathering of siliciclastic sediments in cold environments
Floyd, Cansu (2022-08-31)Dissertation Summary Chemical weathering of silicate minerals is one of the most important Earth processes, moderating atmospheric carbon dioxide levels by consumption of carbon dioxide during hydrolysis of silicates ... -
Characterization and bioinformatic analysis of Pseudomonas aeruginosa condensins
Clevenger, April (2019-12)DNA is organized within chromosomes not only to permit a large amount of DNA to occupy a very small space, but also to serve essential functional roles. Varied levels of organization enable proper DNA replication, repair, ... -
Characterization of a Sporulation Related Two-Component Signaling System in Clostridioides difficile
Morris, Savannah (2023-12-15)Clostridioides difficile is a spore-forming anaerobic human pathogen, that is commonly associated with antibiotic therapy in adults. The disturbance of the gut microbiota, typically caused by antibiotics, allows for an ... -
Characterizing two enzymes involved in carbon utilization and energy production in Syntrophus aciditrophicus strain SB.
Dinh, David (2022-12-16)Methane is both an alternative energy source and a potent greenhouse gas. Biomethanation relies on the metabolic syntrophy between fermenting bacteria, syntrophs, and methanogenic archaea – where large organic molecules ... -
Detailed analysis of protein-DNA interactions driving type II-A CRISPR adaptation
Van Orden, Mason (2020-08)CRISPR-Cas is an adaptive immune system that protects prokaryotes against foreign nucleic acids. Prokaryotes gain immunity by acquiring short pieces of the invading nucleic acid, termed prespacers, and inserting them into ... -
Elucidating the mechanisms of DNA cleavage by CRISPR effector proteins, Cas9 and Cas12a
Parameshwaran, Hari-Priya (2021-05-14)CRISPR-Cas systems are adaptive immune systems protecting bacteria and archaea against intruding genetic elements. Of the different types, the signature nucleases of type II and type VA, Cas9 and Cas12a, are popular choices ... -
Enzyme systems involved in interspecies hydrogen and formate transfer between syntrophic fatty and aromatic acid degraders and Methanospirillum hungatei
Crable, Bryan (2013)The oxidation of either the fatty acid butyrate or the aromatic acid benzoate is essential for the efficient degradation of complex organic material to methane because they are central intermediates. The oxidation of ... -
Exploration of the Vinyl-4-Reductase Effector Domain of the Methanogen Specific Transcription Regulator MsvR in Methanosarcina acetivorans
Mundy-Shelton, Kristen (2017-07-20)Global warming is one of the most pressing issues that this planet faces. Methane production by methanogenic archaea plays a crucial in not only climate change, but also in the global carbon cycle. Methanogenic archaea ... -
Heirloom Microbes: a survey of lactic acid bacteria in traditional Mongolian dairy
Fulton, Shannon (2020)Fermentation has sustained human communities for thousands of years. Through most of that time, humans unknowingly used microbes in food production, especially dairy. It wasn’t until the late nineteenth century that the ... -
THE IMPACT OF LONG-TERM ELEVATED ATMOSPHERIC CARBON DIOXIDE ON BELOWGROUND MICROBIAL COMMUNITY AT CONTRAST NITROGEN CONDITIONS
Liu, Feifei (2017-12)Global terrestrial ecosystems are subjected to various climate change factors, including the concurrent elevated CO2 (eCO2) and nitrogen deposition (eN). Despite the increasing appreciation that eCO2 and eN can interactively ... -
Metabolite Profiling Of Experimental Cutaneous Leishmaniasis Lesions Demonstrates Significant Perturbations In Tissue Glycerophosphocholines
Parab, Adwaita; Thomas, Diane; Lostracco-Johnson, Sharon; Lage de Siqueira-Neto, Jair; McKerrow, James; Dorrestein, Pieter; McCall, Laura-Isobel (2021-05-15)Each year 700,000 to 1.2 million new cases of cutaneous leishmaniasis (CL) are reported and yet CL remains one of thirteen diseases classified as neglected tropical diseases (NTDs). Leishmania major is one of several ... -
Phenotypic, Chemotaxonomic and Genomic Characterization of a Novel Anaerobic Acetogen, Clostridium muelleri sp. nov. P21T Isolated from Old Hay
Doyle, Dena (2020-05)Acetogens are a group of obligately anaerobic bacteria that use the Wood-Ljungdahl (acetyl-CoA) pathway for the production of acetyl-CoA from 2 moles of CO2. Acetyl-CoA is then converted to various acids and alcohols such ... -
Purification, Mechanism, and Biochemical Interactions of the Essential Mycolic Acid Transporters MmpL3 from Mycobacterium tuberculosis, and CmpL1 from Corynebacterium glutamicum.
Stevens, Casey (2020-06)The Corynebacterium – Mycobacterium – Nocardia family (CMN family) are a subset group of actinomycetes which are characterized as acid-fast, Gram-positive species. Many members of the CMN family are causative agents of ... -
SINGLE-NUCLEOTIDE RESOLUTION VIEW OF GENE EXPRESSION IN ESCHERICHIA COLI K-12 UNDER VARIOUS PHYSIOLOGICAL CONDITIONS
Creecy, James (2015-05-08)We analyzed the transcriptome of Escherichia coli K-12 by strand-specific RNA sequencing at single-nucleotide resolution during logarithmic- growth and upon entry into stationary phase under carbon, nitrogen, and phosphate ... -
STRUCTURAL AND MUTAGENESIS STUDIES OF THE YEAST PHOSPHORELAY SIGNALING PROTEINS YPD1 AND SSK1
Branscum, Katie (2015-12)His-Asp signaling systems are ubiquitous in bacteria, archaea, and certain plants and fungi. Little structural information is known about the protein-protein interactions within these signaling pathways, leaving an ... -
Substitutional Analysis of Cysteine Residues and Preliminary Regulon Analysis of the Redox-Sensing Transcription Regulator MsvR in Methanosarcina acetivorans
Gilcrest, Kylie (2016-12)Methane produced from methanogenesis in archaea may be a promising source of renewable energy as methanogens are the primary producers of biogenic methane. However, methanogens are anaerobic and experience transient oxygen ...