Browsing by Author "Gou, Deming"
Now showing items 1-3 of 3
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Identification of rat lung-specific microRNAs by micoRNA microarray: Valuable discoveries for the facilitation of lung research
Wang, Yang; Weng, Tingting; Gou, Deming; Chen, Zhongming; Chintagari, Narendranath Reddy; Liu, Lin (BioMed Central, 2007-01-24)Background: An important mechanism for gene regulation utilizes small non-coding RNAs called microRNAs (miRNAs). These small RNAs play important roles in tissue development, cell differentiation and proliferation, lipid ... -
New design of a lentiviral shRNA vector with inducible co-expression of ARGONAUTE 2 for enhancing gene silencing efficiency
He, Jiening; Huang, Lian; Qiu, Huiling; Li, Jiexuan; Luo, Lan; Li, Yanjiao; Tian, Shengli; Kang, Kang; Luo, Jun; Liu, Lin; Gou, Deming (BioMed Central, 2015-12-08)Background: RNA interference (RNAi) is a robust tool for inhibiting specific gene expression, but it is limited by the uncertain efficiency of siRNA or shRNA constructs. It has been shown that the overexpression of ARGONAUTE ... -
Primer-extension Based Method for the Generation of Sirna/mirna Expression Vectors
Liu, Lin; Gou, Deming; Board of Regents/Oklahoma State University and the A & M Colleges (U.S. Patent and Trademark Office, 2014-08-05)Functional shRNA is produced from an expression vector prepared by selecting a two primer design in which the primers are less than about 50 nucleotides in length, annealing and extending the primers using primer extension, ...