Effect of Serum and Cellular Proteins on Hybrid Vector Transduction Efficiency
Abstract
Our study involved formation of hybrid vector complex from viral and synthetic parts and studying the effect of presence of proteins on transduction of the hybrid vector. We separated proteins from virus-like particles and formed complexes to study their transduction. We added proteins to our viral part and formed complexes and studied the transduction efficiency of the hybrid vector. Our experiments showed that removal of proteins decreased the transduction efficiency of the hybrid vector. We also observed changes in the morphology of complex formed from purified VLPs and PEI compared to that formed from unpurified VLPs and PEI. We concluded that proteins have an impact on hybrid vector complex formation and transduction efficiency. Removal of proteins decreased the transduction efficiency of the hybrid vector and changes the morphology of the hybrid vector.
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- OSU Theses [15752]