Identification of rat lung-prominent genes by a parallel DNA microarray hybridization
Date
2006-03-13Author
Chen, Zhongming
Chen, Jiwang
Weng, Tingting
Jin, Nili
Liu, Lin
Metadata
Show full item recordAbstract
Background: The comparison of organ transcriptomes is an important strategy for understanding gene functions. In the present study, we attempted to identify lung-prominent genes by comparing the normal transcriptomes of rat lung, heart, kidney, liver, spleen, and brain. To increase the efficiency and reproducibility, we first developed a novel parallel hybridization system, in which 6 samples could be hybridized onto a single slide at the same time. Results: We identified the genes prominently expressed in the lung (147) or co-expressed in lung-heart (23), lung-liver (37), lung-spleen (203), and lung-kidney (98). The known functions of the lung-prominent genes mainly fell into 5 categories: ligand binding, signal transducer, cell communication, development, and metabolism. Real-time PCR confirmed 13 lung-prominent genes, including 5 genes that have not been investigated in the lung, vitamin D-dependent calcium binding protein (Calb3), mitogen activated protein kinase 13 (Mapk13), solute carrier family 29 transporters, member 1 (Slc29a1), corticotropin releasing hormone receptor (Crhr1), and lipocalin 2 (Lcn2). Conclusion: The lung-prominent genes identified in this study may provide an important clue for further investigation of pulmonary functions.
Citation
Chen, Z., Chen, J., Weng, T., Jin, N., & Liu, L. (2006). Identification of rat lung-prominent genes by a parallel DNA microarray hybridization. BMC Genomics, 7, Article 47. https://doi.org/10.1186/1471-2164-7-47