Similarity between copper resistance genes from Xanthomonas campestris and Pseudomonas syringae
Date
1993-05Author
Voloudakis, Andreas E.
Bender, Carol L.
Cooksey, Donald A.
Metadata
Show full item recordAbstract
Plasmid-borne copper resistance genes from copper-resistant strains of Xanthomonas campestris pv. vesicatoria from California, Florida, and Oklahoma shared structural similarities. A strain of X. campestris pv. campestris also contained plasmid-borne copper resistance genes similar to the resistance genes from X. campestris pv. vesicatoria. Furthermore, a region of the copper resistance genes from X. campestris pv. vesicatoria 07882 hybridized with copA, the first gene of the copper resistance operon (cop) of Pseudononas syringae pv. tomato. A copper-inducible protein of similar size to CopA was detected by Western blot (immunoblot) analysis from the wild-type strain 07882 and from the cloned copper resistance genes of 07882 introduced into a copper-sensitive strain of X. campestris pv. vesicatoria. A low level of hybridization was observed with chromosomal DNA from other xanthomonads when the copper resistance genes from strain 07882 were used as probes.
Citation
Voloudakis, A. E., Bender, C. L., & Cooksey, D. A. (1993). Similarity between copper resistance genes from Xanthomonas campestris and Pseudomonas syringae. Applied and Environmental Microbiology, 59(5), 1627-1634. https://doi.org/10.1128/aem.59.5.1627-1634.1993